The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
Premium Laboratory glassware Laboratory equipment Chemistry
Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
Premium Plasmid Escherichia coli Agar plate
Investigating the effects of Caffeine on the heart rate of Daphnia Shrimp. Aim: In this experiment we are trying to find out how varying the concentration of caffeine can affect the heart rate of Daphnia shrimp. Prediction: I predict that caffeine will increase the heart rate of the shrimp . Introduction: I believe the results will comply with my prediction. Caffeine is a compound found in especially tea and coffee plants and acts as a natural pesticide. Caffeine is a central nervous system
Free Caffeine Coffee
measurements of systolic arterial pressure‚ diastole arteriole pressure‚ and pulse rate were recorded and observed. The effects of changes in the body were observed and measured during body positioning‚ exercise‚ and cognitive stress. The purpose of this lab experiment was to test multiple variables that can have an effect on blood pressure and pulse. As stated‚ the following variables that were tested were changes in posture‚ exercise‚ and a cognitive stressor that involved spelling forward and backwards.
Premium Artery Blood Blood pressure
Student Name: Amaan Rushdi Lab Partner Name: Dylan Course: BIOL 123 Lab Instructor: Megan Grandinetti Sowbug behavior on environment -lab report What area does the sowbugs prefer to choose for their dwelling? Abstract By working with physical isopods‚ ordinarily known as pill bugs‚ sow bugs or roly- polies‚ we tried whether these sowbugs favored a dark spot to a light place. Included in the lab are a few outlines and tables sketching out our aftereffects of the test. We measured recurrence
Premium Woodlouse Crustacean Experiment
Created By: Andrew Weeldreyer Mrs. Wolf Period:3 AP Chem Lab Report: Determination of the Molar Volume of a Gas Objective: To react Mg and HCL and form hydrogen gas‚ then after collecting lab data‚ determine the hydrogen gas’s molar volume at STP through calculation. Materials: -LabQuest data collector - 3.0 M of HCl solution - Ribbon of Mg metal - Gas pressure sensor
Free Pressure Ideal gas law Gas
Alka-Seltzer Rocket Formal Lab Report Abstract In the Alka-Seltzer Rocket lab one was to experimentally determine how much (grams) Alka-Seltzer and water in mL‚ is needed to launch the rocket the highest/longest distance in cm. Also to experimentally determine the % NaHCO3 in the tablet using the information from the highest/longest distance. Calculate the error and % error of NaHCO3 in an Alka-Seltzer tablet and to determine the limiting reagent for the highest/longest distance traveled. Alka-Seltzer
Free Acetic acid Sodium bicarbonate Vinegar
Zach Sexton December 4‚ 2014 Bot 121 Lab Coleus Lab Report 1. A cutting can form an entire new plant if cutting being used has a meristem. If the cutting has no meristem‚ mitosis will not occur and you will end up with a dead piece of plant material. In order to successfully grow a new plant through a cutting‚ your plant must have adventitious root growth which in order to deliver water and nutrients to the plant. Adventitious roots are created through cell potency where cells differentiate. The
Premium Plant propagation Plant Seed
Lalin Candium Lab Report Chemistry Block H Mr. Neil Chipman Calculating the Atomic Mass for Different Candium Isotopes Objective: In this experiment I will be discovering the atomic weight of Candium isotopes. By doing this experiment‚ it helps us to know how to calculate the relative abundance and atomic mass of each Candium isotopes without having to use the spectrometer. This lab will achieve my understanding of isotopes and why it has different masses but same number of protons due
Premium Chemistry Chemical element Water
Photosynthesis and the Amount of Light _______________________________________________________________________ I. Introduction Photosynthesis the process where plants use sunlight (energy) to synthesize foods forming the products carbohydrates and water (H20 + CO2 + Light CH2O + O2). For photosynthesis to take place they need water‚ carbon dioxide and light and chloroplasts. Light is absorbed inside he thylakoid
Premium Photosynthesis Plant Oxygen