The purpose of this lab is to determine the rate of reaction under different circumstances. Different variables will be manipulated to discover their effects on a particular reaction. Changes in temperature‚ pH‚ and enzyme concentration are examples of factors that have the potential to affect the initial rate of an enzyme catalyzed reaction in a controlled experiment‚ whether it be speeding the reaction up or slowing it down. Part I of the experiment establishes a baseline that can be used to compare
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
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Triphosphate (ATP)‚ which gives them the energy they need to perform. In this experiment‚ creatine is added to water and its effects on Daphnia is tested. By observing the heart rate‚ one can determine if the creatine increases or decreases heart rate. Heart rate was chosen to be measured because one can then see how creatine affects the heart. If it is unhealthy for the Daphnia‚ it could potentially be unhealthy for humans. “Long-term safety of creatine supplementation has been questioned. This retrospective
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Lauren Sullivan Plants Imperfections Abstract The purpose of this experiment was to plant different crosses and observe the different phenotypic ratios the plants present. The procedure of this experiment was plant six different crosses and water them correctly so that we could observe the different phenotypes and compare them to Mendel’s proposed ratios. Mendel‚ who had studied peas‚ did a similar experiment and came up with specific ratios that a monohybrid and dihybrid cross should show. His
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Question: How does the number of sit-ups affect your heart rate. Independent Variable (IV): Number of sit-ups ( 0‚5‚10‚15‚20). I chose these 5 increments because they are consistent numbers in which everyone can participate without it being to much. Dependent Variable: heart rate( BPM) +/- 4 ( number of beats in 15 seconds multiplied by 4). Materials: timer‚ flat surface‚ movable clothing Background: In this experiment I will test your heart rate and if it increases or decreases with exercise (sit-ups)
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the 10-12 year old subject’s heart rate read 83 beats per minute (bpm). After doing jumping jacks the 10-12 year old subject’s heart rate read 96 bpm‚ that is a difference of 13 bpm. Before doing jogging‚ the 10-12 year old subject’s heart rate read 82 bpm. But after doing jogging for two minutes‚ the 10-12 year old subject’s heart rate read 92 bpm‚ that is a difference of 10 bpm. As it is shown in my data table‚ before doing sit-ups the 10-12 year old subject’s heart rate read 72 bpm. After doing sit-ups
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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