The Effects of Using the Heat Shock Treatment to Deliver a Vector in Genetic Transformation Introduction: In this experiment we are testing what is required for E. coli to successfully grow on LB (Luria Broth) plates with ampicillin and determining if any genetic transformation has occurred. By combining +pGLO LB and ampicillin we should get an ampicillin resistant gene and by using –pGLO we should create a non-genetic resistant bacteria. The pGLO plasmid has the GFP (green fluorescent protein)
Premium DNA Gene Genetics
The intrinsic Conduction System 1. List the elements of the intrinsic conduction system in order starting from the SA Node. SA node‚ AV Node‚ Av Bundle‚ left and right bundle branches‚ Purkinge fibers At what structure in the transmission sequence is the impulse delayed? Av Node Why? Allows completion of atrial contraction before initiation of ventricular systole. 2. Even though cardiac muscle has an inherent ability to beat. the nodal system plays a critical role in heart physiology. What
Premium Heart Cardiology
The Answer by Karl Nemsow Voyager1 sails through deep space all alone. It has been on its journey since 1977 sending back glimpses of the solar system to help humankind find some answers. One of the greatest questions asked by humankind is what‚ or who‚ else might exist out in our grandiose cosmos. A cosmos 13.7 billion years old‚ which is so massive that it is incomprehensible to the human mind. Dr Vince Hawking tried to comprehend such concepts every single day. It made him wonder if
Free NASA Space exploration Kennedy Space Center
Medicine in Sport (2008) 11‚ 6—19 REVIEW Heat stress and strain in exercise and sport John R. Brotherhood The University of Sydney‚ Exercise and Sport Science‚ Faculty of Health Sciences‚ Australia Received 3 May 2007 ; received in revised form 27 August 2007; accepted 28 August 2007 KEYWORDS Heat stress in sport; Exercise thermoregulation; Rational analysis of heat stress; WBGT; Risk assessment for exertional heat illness Summary Heat stress arising from the thermal environment is of
Premium Temperature Heat transfer Heat
Title: Specific Heat Capacities of Metals Experiment Date: 3/28/2012 Report Date: 4/4/2012 Purpose: The purpose of the lab is to measure the specific heat capacities of aluminum‚ steel and brass. Theory: The amount of heat (ΔQ) required to change the temperature of an object is proportional to the mass (m) of the object and the temperature change (ΔT) of the object. ΔQ= cmΔT where c is called as the specific heat capacity of the material. In the calorie units system the unit of quantity
Premium Heat Specific heat capacity Thermodynamics
"The Answer is No" Historical content is not necessary for the reader to understand the short story "the Answer is No" by Naguib Mahfouz. It is possible to understand the theme of the story without knowing the author’s background or the story’s historical context. "The Answer is No" is about a fourteen year old girl who gets sexually assaulted by her new tutor who is of the same age as her father. The tutor tries to convince her to marry him in exchange for her education at a prestigious College
Free Human sexual behavior Sexual intercourse Rape
The Answer Is No From centuries to centuries the role of women has been predominantly subordinate to the role of men. Men have been known to be superior in both physical and mental levels. They are also most likely to have an "upper hand" in situations based on their gender. An example of such situation is taken place in "The Answer Is No"‚ written by Naguib Mahfouz. This story has a considerable amount of sexual politics. On one side you have a women who is powerless and feels trapped because of
Free Woman Sexual intercourse Gender role
MA‚ PAULINE Date Performed: 10 SEPTEMBER 2014 NACIONGAYO‚ DANIELLE Date Submitted: 17 SEPTEMBER 2014 TEDERA‚ YVES HEAT EFFECTS AND CALORIMETRY Experiment No. 2 I. RESULTS A. Determination of Heat Capacity In this experiment‚ an improvised calorimeter was used to determine the heat capacity. The calorimeter weighed 4.47 grams prior to the addition of water. Tap water‚ 40 mL to be exact‚ was added to the calorimeter which increased the weight to 43.87 grams. The water was measured using
Free Thermodynamics Enthalpy Energy
language. Use visual cues‚ pointing to things to show what you mean. Approach the individual from the front. An unexpected touch or drawing near from behind may startle and upset the person. Ask only one question at a time and allow time for an answer. If he does not seem to understand‚ repeat the question using the same wording. If this does not work‚ after a few minutes‚ rephrase it. Eliminate distractions‚ such as the TV or radio‚ when talking to the person with dementia. Use humour whenever
Free Communication Person Interaction
conducted was to analyse the work of a heat exchanger‚ by studying the effects of heat transfer from a hot stream and a cold stream in the heat exchanger. In the experiment we were introduced to the shell and tube heat exchanger and were told of all its parts and how to use them. The experiment started by evaluating what the heat transfer rate for a hot stream and a cold stream was within the heat exchanger. Using this information the experiment went on to analyse the heat exchanger in more detail. The log
Premium Heat transfer Heat exchanger Heat