Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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to break up any large pieces of the substance by pressing the pieces against the wall of the crucible. Measure and record the mass of the crucible with compound. 3. Set up a ring stand‚ ring‚ and clay triangle for heating the sample. Rest the crucible on the clay triangle. Set up a lab burner and ignite the burner away from the crucible. Adjust the burner to get a small flame. 4. Hold the burner in your hand and move the flame slowly back and forth underneath the crucible to gently heat the sample
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The Mass of Acetylsalicylic Acid in Aspirin ------------------------------------------------- Purpose The purpose of the lab is to determine the mass of the ‘active ingredient’ in a commercial ASA tablet. ------------------------------------------------- ------------------------------------------------- Introduction There are three main theories surrounding acids and bases including the Arrhenius‚ Bronsted-Lowry‚ and Lewis theories. The Arrhenius theory of acids and bases states that
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Lab Report 1: Natural Selection Introduction: In today’s lab we learned how natural selection and survival of the fitness took place in Darwin theory by testing it out with four different utensils which were Spoon‚ Fork‚ chopstick‚ and clothespin which represent the four different types of bird beaks. Our goal was to see which one utensil ( bird beak) would outcome the other four utensil ( bird beak) by being able to survive with the limit amount of food‚ the environmental chances‚ and see who
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The refraction of light lab used common materials to examine the process of the refraction of light. The title of this lab is exactly what occurs throughout the four different experiments. All four parts of this lab use prior knowledge to observe the complex concept of refraction. Refraction is the bending of a light when it enters a medium where the speed of light is different. When light enters through the glass and water the speed of light becomes decreased. This is due to the density of water
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Lab Report: Scientific Method Your Name: Alyssa Melincoff Purpose of this Lab What is the goal of this lab? What question is it trying to answer‚ or what problem is it trying to explain? To find out how table salt affects the boiling temperature of water. Hypothesis After reading the lab instructions - but before starting the lab - record your best “educated guess” about what will happen in the experiment. What hypothesis can you generate about the effect of adding salt to water? Adding
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2218 Electronic Systems and Design HLab02: Op-amp Schmitt Trigger “In this lab‚ we will investigate Schmitt trigger comparator circuit. ” “The pre-lab reading will help you make sense of what you are about to do in the lab. The lab tasks and evaluation and review questions will form the basis of your lab report. See course document on how to write the lab report.” 1.0 Lab Learning Outcomes After completing this lab‚ students will be able to:1. Compare the input and output waveforms for a
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Name:Date:Professor: Converting Decimal to Binary and Binary to Decimal (20 points) Instructions for the Binary Conversion Exercise: NOTE! LOG ON TO SKILLSOFT TO DOWNLOAD THE LAB INSTRUCTIONS AND START THE LAB. In computers binary code is the language that communicated between applications. Binary Code is a coding system using only digits 0 and 1 to represent a letter‚ digit or other characters in a computer. It is hard to imagine that huge and hard calculations are done on computers applications
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Observing and Analyzing the Behaviors of Daphnia‚ Pill Bugs‚ Amoeba and Uglena Under Certain Experimental Conditions Testing Reactions to Gravity‚ Light and Food Jacob Kluth and Kelsey Jankowski Introduction This lab has introduced the use of daphnia‚ pill bugs‚ amoeba and Uglena. We the AP Biology students were first introduced to the test subjects to test his or her capability of handling living organisms for upcoming science experiments. The AP Biology students were asked to create original
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and familiarize ourselves with their taxonomic levels through the organism’s mechanisms of feeding‚ motility‚ and reproduction. Our group used the prior knowledge we possessed about the Hydra‚ Rotifer‚ Gammarus‚ and Planaria organisms to complete the lab. We knew that all of these small invertebrates have a freshwater habitat. Each organism differs in taxonomic levels from Genus to Species. We knew that the Hydra reproduces with one of two methods: 1) by “budding” which is an asexual method of reproduction
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