FACT: Potassium tellurite (K2TeO3) inhibits the growth of gram-negative bacteria and most of the upper respiratory tract normal flora. Oxyanions of tellurium‚ like tellurite (TeO32-)‚ are highly toxic for most eukaryotic and prokaryotic organisms. Known for its antimicrobial properties‚ potassium tellurite has been used in selective media for the isolation of a number of naturally tellurite-resistant bacterial species such as the C. diphtheriae. These tellurite-resistant bacteria reduce tellurite
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Materials and Methods: Inoculation of the unknown was done on a nutrient slant agar The nutrient agar was then incubated at 27°C for one week. The agar appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain
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Agar From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Not to be confused with auger or augur. For other uses‚ see Agar (disambiguation). Culinary usage Mizuyōkan - a popular Japanese red bean jelly made from agar. Scientific usage A blood agar plate used to culture bacteria and diagnose infection. Agar or agar-agar is a gelatinous substance derived by boiling[1] a polysaccharide in red algae‚ where it accumulates in the cell walls of agarophyte and serves as the primary structural
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours. Upon observing the growth on this plate‚ it is fairly obvious that one of the organisms is Serratia marcescens. However‚
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ways we can differentiate the different species. Looking at these bacterium underneath the microscope is an important step to determine which kind of species it is. All cocci bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium found in humans. It is frequently found on the mucus membrane in the nasal cavity and on the skin. It also infects many people in the hospital‚ also called
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no indentation. 3. Describe the appropriate specimen collection procedures‚ staining methods‚ and culture techniques used for isolation of yeast. Collection procedures Specimen of choice include respiratory secretions‚ hair‚ skin‚ nails‚ tissue blood or bone marrow and CSF. Swabs are inadequate. CSF: Concentrate by centrifugation before inoculation. Make India ink preparation or latex agglutination for Crytococcus neoformans with part of the specimen and then inoculate the remainder onto
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Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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Problem What effect does the different concentration of hydrochloric acid have to the rate of diffusion of agar that is dissolved with sodium carbonate and phenolphthalein? Introduction Diffusion is a process where the molecules intermingle as a result of their kinetic energy of random motion and is the net movement of like molecules down their concentration gradient‚ which is the energy inherent in their constant molecular motion makes them collide and careen outward from the region of higher
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staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during the activity in lesson proper(flashcards) * iodine-
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