Gram Stain: Gram is an empirical method to distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the
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Diffusion in agar cubes Introduction: All cells are dependent on a processes called diffusion‚ which is the movement of a substance from an area of higher concentration to an area of lower concentration. Cells need to obtain certain essential substances‚ like oxygen‚ for their survival. The rate of diffusion is said to be dependent upon the various factors: Size of molecules that have to diffuse‚ concentration gradient‚ the distance across which diffusion occurs‚ number of pores/openings‚ surface
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T-φ gram Determination of Unreported Meteorological Quantities from T-φ gram 1. Mixing Ratio (W):- It is the ratio of the mass of water vapour to the mass of dry air containing the vapours at a certain temperature. It is expressed in gm/kg. On T-φ gram from dew point temperature we follow isohygric doted green line and read the value at the base. (It can be calculated at all levels where dew point temperature is available). 2. Saturation Mixing Ratio (Ws) :- Mixing ratio at
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Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive
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Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian’s gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria
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February 2011 Vol 55 Chief Editor : Neeta Prasad Joint Director (Prod) : J.K. Chandra Editor : Manogyan R. Pal Cover Design : Manjula Das E-mail (Editorial) : editoryojana@hotmail.com : yojanace@gmail.com (Circulation) : pdjucir_ jcm@yahoo.co.in Website : www.yojana.gov.in Let noble thoughts come to us from every side Rig Veda CONTENTS Grassroots Level Democracy in India : An Assessment...........................................................................5 Mathew
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growth of bacteria on Agar plates Hypothesis: That the bacteria will grow in colonies throughout the agar plates except for the one with the anti-biotic loop because some will fight off the bacteria. Method: (Method taken from prac sheet) Plate 1: Use the swab to cover your entire agar plate in your bacteria. You only need one swab of bacteria but be careful to cover the entire surface of your agar in a layer of bacteria. Carefully place an Antibiotic Mastring on top of the agar (use tweezers) in the
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Peradeniya Functions of Blood * Blood is pumped by the heart through blood vessels‚ which extend throughout the body. * Blood helps to maintain homeostasis in several ways. 1. Transport of gases‚ nutrients‚ and waste products. * Oxygen enters blood in the lungs and is carried to cells. * Carbon dioxide‚ produced by cells‚ is carried in the blood to the lungs‚ from which it is expelled. * Ingested nutrients‚ ions‚ and water are transported by the blood from the digestive tract
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Biology Assessment: Influenza Structure: Viruses are non-cellular obligate intracellular parasites‚ requiring a living host cell in order to reproduce. A developed viral particle (virion) lacks the metabolic machinery of cells‚ containing just a single type of nucleic acid (DNA or RNA) encased in a protein coat or capsid. Viruses can be distinguished by their structure and by the nature of their genetic material (single or double stranded DNA or RNA). Viruses that affect humans are more difficult
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