The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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Most important scientific discovery by mankind ever Geoffrey Macharia Maseno University Most important scientific discovery by mankind It is a fact that science continues to play a significant role in the existence of mankind and the extent to which he relates to the universe. Man has continued to make great scientific discoveries that have made it possible for him to exploit most of the natural resources to his advantage. Increase in world population has led to a major
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and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the
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Enzymes: Food & Nutrition What are enzymes Enzymes are a type of protein produced by a living organism used to catalyze chemical reactions in cells. These reactions allow the cell to build things or take things apart as needed in order to grow and reproduce. How do enzymes work - in steps 1) Substrate floats near enzyme 2) Substrate and enzyme connect – which breaks it into products 3) Products are released ex) BreadFast & Co.’s use of enzymes The company uses many different
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Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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Abstract The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor
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Biology‚ Section G 27 September 2013 Experiment 5.3 Title: The Fragility of an Enzyme Purpose: To see how easily enzyme function can be destroyed. Hypothesis: I think that the bowl with only Jell-O will set as will the bowl with heated pineapple but the Jell-O with fresh‚ uncooked pineapple will not set. Materials: Part of a fresh pineapple A blender or a fine cheese grater 3 small bowls A small box of Jell-O Pot Stove Refrigerator 2 tablespoons Procedure: 1. Cut the pineapple
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Temperature affecting enzyme activity Aim: To see how temperature can affect enzyme activity Prediction:-I think the one that will work best will be the rennin placed in milk at 37oC My independent variable will be the temperature My dependant variable will be how thick the milk becomes The equipment I need is milk‚ 5 test tubes‚ rennin‚ water bath‚ boiled rennin‚ a glass rod and a stopwatch Introduction: Rennin is an enzyme found in the stomach‚ its function is to solidify milk. We
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Enzymes are catalytic proteins which speed up the rate of reactions. Every enzyme has a specific function – meaning‚ they can only bind to certain substrates. Because these enzymes are proteins‚ they can be denatured. Enzymes can be denatured by many factors‚ such as pH and temperature. This lab was divided into three parts which examined the effects of pH‚ enzyme concentration and temperature on the rate of which enzymes catalyze. The pH is an index of hydrogen ions. In acidic conditions‚ where
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Drug discovery is a process in medicine and pharmacology where a drug is discovered and designed. In the past drug discovery was very different to the current form‚ previously active ingredients were used to tackle illnesses. Nowadays more detailed research on diseases has been pursued‚ details such as what the genes are coding or what protein is involved‚ therefore are allowing the designing of drugs to target specific sites to act on the disease. Once detailed information of the disease has been
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