1. A) I would expect the active site of nylonase to consist mainly of polar amino acids with a few nonpolar amino acids as well because the substrate for nylonase is polar overall‚ but has many nonpolar bonds. What makes me think that the nylonase enzyme is polar is that the substrate that would bind to the active site of nylonase has extreme polarity between carbon and oxygen‚ and between hydrogen and nitrogen due to their differences in electronegativity’s‚ but it still has the nonpolar bonds between
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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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This experiment was performed in order to analyze the effects of pH‚ enzyme concentration‚ and temperature on the catalytic rate on enzyme ALP. The initial experiment was done to approximate the optimum pH for the activity of the enzyme. The hypothesis was that alkaline phosphatase activity will be critically affected by pH effects. The hypothesis was valid because the data shows that the pH values for the acidic‚ basic‚ and neutral tubes increased over time‚ absorbing at similar rates. The basic
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Potato Enzyme Lab Enzymes are protein catalysts that speed up chemical reaction in living organisms. The purpose of this lab was to; observe the breakdown of hydrogen peroxide toxin by potato’s enzyme catalase‚ determine factors that influence how quickly the reaction takes place‚ and determine factors that influence how well enzymes function. The first test‚ we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added
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Enzyme Lab Report Introduction The objective of this experiment was to determine if changes in pH or temperature affected the activity of enzymes‚ specifically the enzyme sucrase. Enzymes are protein molecules that act as biological catalysts to increase the speed of the reaction or to lower the activation energy of that reaction. However‚ the activity of an enzyme can be affected by physical factors such as pH and temperature because these factors alter the structure of the enzyme (Freeman‚ 2011)
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Enzyme-Controlled Reaction Procedure: Click the TV/VCR. Then click the Play button on the video controller. Watch an animation about enzyme action. Click More Information to read about enzymes and substrates. To conduct the experiment: Adjust the pH level of the test tube by click the up and down arrows Add substrate to each of the test tubes that already contain an enzyme solution Click and drag a piece of weighing paper with the powdered substrate to a test tube. Click the computer monitor to
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In this enzyme lab‚ (insert creative name)‚ the enzyme catalase was observed under varying conditions in order to interpret what prohibits and inhibits the functioning of certain enzymes in chemical reactions. Enzymes aid in chemical reactions by speeding up the time it takes for the reaction to occur‚ without getting “used up” throughout the process. Catalase‚ the specific enzyme used in this lab‚ is a protein abundant in the liver and red blood cells. It enhances the breakdown of hydrogen peroxide
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The experimental results were different from the hypothesis because while the enzyme appeared to not work as well‚ I expected a more significant change. Most of the time‚ there was only a millimeter of difference of the foam between the two samples while I expected a greater difference such as 10 millimeters. Enzymes speed up chemical reactions and at the active site‚ a substrate can be broken down or two substrates can form a larger molecule. Hydrogen peroxide is broken down by peroxidase into water
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HINTS ON WRITING YOUR REPORT Title: Enzyme activity Objective: To investigate the effect of temperature on amylase activity Design principle Background: Amylase activity products? (show the equation) Which factors will affect enzyme activity? How to study the rate of reaction? (e.g. rate of disappearance of substrates or rate of formation of products) Independent variable: temperature of reaction mixture or at which the enzymatic reaction occurs. It can be varied by setting water bath at
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Lab Report Enzymes (must be typed) Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL What is the Hypothesis of this experiment? (3 points) -The highest temperature will affect the reaction rate of enzymes. What is the dependent variable? (1 point) -Change of rate/product/color/absorbency. What is the independent variable? (1 pt) -The temperature. Explain in detail the procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times)
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