pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil‚ and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is
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Effect of pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st‚ 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body‚ enzymes
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discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic‚ the catecholase
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The effect of time on enzyme reaction. Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds‚ there would be the least amount of H2O2. The enzyme activity mirrored my predictions
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Biology Controlled Assessment. Kenishia Pascal 10x3. Investigating How Different Concentration Effects The Rate Of Reaction. Strategy A Possible Factors * Source of catalase * Concentration * Surface Area of enzyme * Concentration of enzyme * pH * Temperature Chosen Factor We chose to investigate the concentration of enzyme as we had previously investigated the optimum temperature for catalase in the preliminary investigation. Concentration of enzyme is also
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Enzymes are proteins or nucleic acids that catalyze reactions. They are able to speed up reactions by reducing the activation energy of a reaction. Each kind of enzyme has a specific shape that matches its substrate so it can bind to its active site. Enzymes convert their substrates into a product. Enzyme activity are affected by factors such as temperature‚ pH‚ and time. If an enzyme is exposed to extreme heat‚ it will become denatured‚ that is‚ to become deformed and lose its original shape which
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Interpretation of Data When looking at my results i can safely say that the change in HCl concentration does affect the rate of reaction with the Calcium carbonate pebbles. The relationship between the change in HCl and reaction rate can be seen in the graph and raw data table presented above. If you compare the lowest concentration with the highest concentration you’d be able to see that there is a very large contrast between their averages. The average gas production of 0.1M HCl was 0.00693 kPa/s
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Does concentration affect the speed of chemical reactions? Problem: We are proving that “concentration” (the amount of reactants) is one of the things that can affect the speed of chemical reactions. We are also finding a way to keep the lid on the film canister for the longest period of time. We are using vinegar and water as reactants and changing the concentration every time to see the difference between the rates of reactions speed. Materials: - 1 graduated cylinder - 1 film canister
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Enzymes are biological catalysts that speed up chemical reactions by lowering the amount of activation energy needed to start the reaction and let the reaction occur at temperatures found in living cells. The way that enzymes do this is explained with the lock and key hypothesis. This hypothesis says enzymes have a specific shape called the active site which is different between different enzymes. Molecules called the substrate that participates in the reaction also have a specific shape that can
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