investigate the effect of temperature on the enzyme catalase. The original research question was exploring the effect temperature would have on a yeast catalase reacting with hydrogen peroxide (H2O2). To address the latter question a series of experiments were conducted. The various temperatures experimented with were as follows: 22 degrees Celsius (room temperature)‚ 0 degrees Celsius (freezing)‚ 100 degrees Celsius (boiling)‚ and 37 degrees Celsius. Along with variations in temperature‚ the experiment
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THE EFFECT OF TEMPERATURE ON CATALASE RESEARCH QUESTION: What is the effect of various temperatures‚ 0°C‚ room temperature‚ 37°C‚ 50°C‚ 60°C‚ on the number of oxygen gas bubbles liberated‚ in a decomposition reaction between the enzyme Catalase‚ obtained from crushed mung beans‚ and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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Investigate factors affecting cell membrane permeability in beetroot. Research Question: How does variation in temperature affect the permeability of cell membranes? Background Information: In this investigation I will want to identify how variations in temperature effect the permeability through the use of a beetroot. I predict that as the temperature of the water increases so will the dispersion of the pigment. Independent Variable: The temperature of water will be increased in increments of 10oC
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Methods We began this experiment by turning the Spectrophotometer to 605 nm absorbance‚ and setting up each of the tubes as stated in Table 7-2*‚ ignoring the DPIP until the rest of the sample was set up. Once all of the samples were set up accordingly‚ we blanked the spectrophotometer‚ and added the DPIP to the sample right before‚ inverting the sample and taking a reading at the Zero time mark‚ and placing the sample in the designated light apparatus‚ Sample 1 was wrapped in aluminum foil to
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How does lava temperature impact viscosity and velocity? The higher the temperature the lower the viscosity which allows it to develop a smooth surface skin‚ but this is quickly broken up by flow of molten lava‚ it creates a rough‚ clinkery surface. The lower the temperature of the magma the higher the viscosity which allows the lava to travel down the slope. In the group the average velocity was 0.91 cm/s . How the “lava” flowed was in a high viscosity and a low velocity because the “lava” was
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Two experiments were performed to investigate the effects of temperature on the growth rate pine seedlings. Experiment 1 A batch of 60 pine seedlings (Batch I) was grown in a greenhouse under controlled temperatures. Ten plants were grown at each of six temperatures from 50 C to 300 C. These plants remained at their original temperatures 24 hours each day for two weeks. All other conditions were the same for all seedlings. The growth rates are shown in Table 1. Table 1. Growth rates for pine
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apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful‚ ensuring they don’t come into contact with our eyes‚ skin or clothes. Catalyse is an enzyme found in all living cells. It makes Hydrogen Peroxide decompose into water and Oxygen. We will be measuring the amount of Oxygen released from the Hydrogen Peroxide. In order to do this we will use a measuring cylinder. This piece of apparatus measures the amount of
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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