EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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CHAPTER I INTRODUCTION Background of the Study The use of plants for remedies has long been in existence and is among the most attractive sources for developing drugs (Bukantis et al.‚ 1980). Any part of plant can be considered as herbs including leaves roots‚ flower‚ seeds‚ resins‚ leaf sheath‚ bark‚ inner bark (cambium)‚ berries and sometimes the pericarp or other portion (Holetz et al.‚ 2002). Most primates depend heavily on the leaves‚ fruits‚ and flowers of tropical plants to meet their nutritional
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DNA Extraction from Fruit 1. What was the purpose of adding liquid soap and salt in step #1 and how does NaCl contribute to maximum DNA extraction. The purpose of using soap was to destroy the membranes inside a kiwi cell. Soap helped with that because it dissolves the membranes easily. Salt or NaCl was used to remove proteins and carbohydrates. NaCl caused the proteins and carbohydrates to precipitate. 2. Why was it necessary to “mush” the kiwi by hand? If the step was omitted‚ what
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Harmful effects of smoking Doan Thi Huong Thao BAIU08155 International University HCMC Academic English 2 Bien Thi Thanh Mai Instructor May 17‚ 2010 Abstract Smoking is known to be a primary cause of harmful effects on health‚ family‚ environment and society. However‚ scientist research in health and environment‚ that researches show that smoking cause many diseases‚ even lung cancer. An aim of my study has been to determine that smoking also effect on family and society. Results indicated
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GENETICS (DNA - CHROMOSOMES) 7/16/2013 DNA – Chromosomes - Genes DNA •DNA: the chemical inside the nucleus of a cell that carries the genetic instructions for making living organisms. •The material inside the nucleus of cells that carries genetic information. • The scientific name for DNA is deoxyribonucleic acid. D.J.A 1 7/16/2013 http://www.ncbi.nlm.nih.gov/ Chromosomes • A chromosome is one of the threadlike "packages" of genes and other DNA in the nucleus of a cell. •
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1. Sequence A TCT TCC CTC CTA AAC GTT CAA CCG GTT CTT AAT CCG CCG CCA GGG CCC CGC CCC TCA GAA GTT GGT Sequence B TCA GAC GTT TTT GCC CCG TAA CAA CTT GTT ACA ACA TGG TCA TAA ACG TCA GAG ATG GTC AAT CTC TTA ATG ACT Sequence C TAC AAA CAT GTA AAC ACA CCC TCA GTG GAC CAA CTC CGC AAC ATA AAC CAA ACA CCG CTC GCG CCG AAA AAG ATA TGG 3. 4. Sequence A - Middle Sequence AGA AGG GAG GAU UUG CAA GUU GGC CAA GAA UUA GGC GGC GGU CCC GGG GCG GGG AGU CUU CAA CCA Sequence B - End Sequence
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it and teachers say that parents demand more of it. Teachers assign homework to help some students improve their grade and pass the course for those of who do not do well on tests or standardized examinations. Can homework be considered helpful or harmful to students? This controversy turns into arguments and disagreements. For me‚ homework totally can help students in study because there are many benefits we can get from doing homework. Everyone has their own opinion right? I have my own opinion too
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------------------------------------------------- Be Wise With Plastics Plastics are everywhere and in most cases are very affordable and convenient. But‚ increasingly scientists are finding that a hidden cost may be our health. Some common plastics release harmful chemicals into our
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in electrostatics. The electrostatic force between two charges of 200 µC and 500 µC placed in free space is 5 gf. Find the distance between the two charges. (g = 10 m/s2) 12. Derive an expression for the electric current in terms of drift velocity. How the drift velocity of the electron depends on the temperature of
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scale. Since there‚ there have been numerous findings about harmful effects of smoking cigarettes. They affect three problems: health‚ family and environment and society. However‚ according to Richmond (1994)‚ nicotine produces a good effect for the individual in a way that it helps people relieve stress and generates a certain “calming effect”. Besides‚ Hollywood and other media productions associate smoking with manliness‚ and maturity Harmful effects of smoking on our health Every year thousands
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