B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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How Guns Work A gun is a weapon that uses the force of an explosive propellant to project a missile. Guns or firearms are classified by the diameter of the barrel opening. This is known as the calibre of the gun. Anything with a calibre up to and including . 60 calibre(0.6 inches) is known as a firearm. The precise origin of the gun is unknown‚ although they were in use by the early 14th century and were common place in Europe by mid-century. These early guns were nothing more than large
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My point of view on this matter comes from experience at the prison. For something to work‚ the person has to use it first. By just buying a vacuum cleaner doesn’t make the house clean. The individual has to use the vacuum cleaner to clean the house then it will be clean. The same idea with the inmates‚ by just providing the programs and classes doesn’t make them better. There a lot of programs that are offered inside a prison that inmates can take advantage of but there are multiple problems as
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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How to make a coil pot 1.Before you get started‚ mix a little clay and water together in a container. Stir it until it is nice and slimy. This gooey mixture is called slip. 2. It sounds backwards‚ but to make a coil pot‚ you start from the bottom. Pat a piece of clay into a round flat disc about as thick as a pencil. Try to make it as perfectly round as you can. You can trace around the bottom of a glass if you need help forming the clay into a round shape. Make your base at least three inches
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Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
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popularity‚ and love and so on and so forth. With this said‚ does this mean that having less of them will not make anyone happy? Unfortunately‚ this is not so. Real happiness is something that a person brings upon to himself and should never depend on what the world can or cannot give him. For those who want to achieve happiness‚ read on a little more and learn about the secrets on how to find happiness in your life‚ today. Find love for yourself Start from the beginning. You simply cannot give
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An Investigation into How a Pendulum Works Isaac Olson Mitch Koebnick‚ Kyle Orr 13 September 2013 A Period Purpose There are three purposes of this experiment. The first purpose is to determine the relationship of the period and the amplitude of the pendulum. The second purpose is to determine the relationship of the period and the mass of the pendulum. The final‚ but most certainly not the least‚ purpose of this experiment is to determine the relationship of the period and the length
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An Le Foundation Biology lab BRADFORD PROTEIN DISCUSSION ESSAY The appearance of blue color showed the present of protein in the BSA dilutions. The more diluted the solution was‚ the less blue it was. The R2 value of the standard curve of BSA dilution was obtained to be 0.9972‚ which is close to 1. The closer to 1 the R2 value was‚ the more accurate the linear portion was. The error percentage of each unknown was large: 25.9% for skim milk‚ 95% for soy sauce‚ and 64.7% for egg white. The vast
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Question 1: How does Osmosis works It refers to the movement of water molecules from an area of high-level water concentration‚ that is‚ a dilute solution‚ to an area of low-level water concentration‚ that is‚ a strong solution through a semi-permeable membrane. (Passmyexams.co.uk‚ 2015) Actually‚ water moves in a bi-directional manner with the aim of leveling up the concentration. However‚ a high number of water molecules move from the fresh water towards the salty water. The outcome
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