Na’imat. Reg. No. : 0105514. Course: Immunology laboratory. Instructor: Dr. Muna Hassuneh. Report subject: Serum protein electrophoresis. Report No.: 1. Serum Protein Electrophoresis (SPEP) Introduction: The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. And its uses an electrical field to separate the proteins in the blood serum into groups of similar size‚ shape‚ and charge. And here we’ll use gel electrophoresis which
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EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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Cheyenne Kenny September 29‚ 2014. Biology Properties Of Water Adhesion How does temperature change affect surface tension? As the temperature of a liquid increases‚ its surface tension decreases. When water heats up‚ the movement of its molecules disrupts the imbalanced forces on the surface of the water and weakens its sheet-like barrier of tightly bound molecules‚ thereby lowering the surface tension. This is why hot water is more effective when cleaning; its low surface
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In our experiment‚ we will be testing three different proteins to see if they denature at the same temperature. A protein is a molecule containing amino acids created in ribosomes that have many functions‚ including being an enzyme and transporter‚ structuring‚ translating genetic information‚ and serving as a channel for charged molecules to pass through the cell membrane. As a molecule‚ a protein is shaped like a coil (Food Science Basics 2014). Denaturation is when a protein’s shape changes due
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selection down to two choices: (1) Franchise L‚ Lisa ’s Soups‚ Salads‚ & Stuff‚ and (2) Franchise S‚ Sam ’s Fabulous Fried Chicken. The net cash flows shown below include the price you would receive for selling the franchise in Year 3 and the forecast of how each franchise will do over the 3-year period. Franchise L ’s cash flows will start off slowly but will increase rather quickly as people become more health conscious‚ while Franchise S ’s cash flows will start off high but will trail off as other
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Relationships of Proteins Proteins are a group of organic compounds whose molecules consist of carbon‚ hydrogen‚ oxygen‚ nitrogen and sometimes sulfur atoms. They are condensation polymers of amino acids that are joined by strong peptide bonds. There are 20 different types of amino acids available‚ and the different combinations of these amino acids give it different structures and specific functions to it. Below in this essay‚ will illustrate the how different structure of a protein is related to
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Task 1 • Describe the structure of an enzyme as a protein‚ in terms of tertiary/ quaternary structures. 1) Primary Structure This is in reference to the order of way that amino acids are connected to form a protein. These are built up from 20 amino acids‚ and follow these structures o A carbon (the alpha carbon) bonded to the four groups below: o A hydrogen atom (H) o A Carboxyl group (-COOH) o An Amino group (-NH2) o A "variable" group or "R" group 2) Secondary Structure This is in reference
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specialised to suit a specific purpose. An example of this is the pancreatic beta cells found in the islets of Langerhans. These cells are specialised to synthesise the protein insulin that is involved in the metabolism of glucose in the cells. (Layden‚ 2010) Figure 1 - Picture to show basic structure of a cell. (Farrabee‚ M J. 2007) But how is insulin made? The production of insulin starts in the nucleus which is the cellular organelle where the DNA (deoxyribonucleic acid) is found. They form a series
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to three pages (2 full pages minimum) 6. double spaced 7. 12 point font 8. 1 inch margins on all four sides (this may require you to manually change the default settings on the Word processing software) 9. ✓Don’t forget‚ you are also learning how to write in this class. Proofread Proofread Proofread! 10. ✓Do review the documents on writing in the Student Tips folder of the Resources section prior to beginning your essay. These writings should NOT be summaries of the video‚ but your reactions
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How Technology Can Assist or Hinder Information Management Practices in Organisations 1 INTRODUCTION Technology has come a long way in the past 10 to 15 years and we now have access to information at virtually any time‚ or place we wish. Organisations today rely heavily on technology and without it‚ would likely struggle to survive. Technology has enabled us to better service the needs of consumers in the ever-changing market. 2 ADVANTAGES The obvious advantage with technology today is
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