Analysis of protein post-translational modifications using DIGE-based proteomics Robert M. DeKroon‚ Jennifer B. Robinette‚ Cristina Osorio‚ Sun Yong Jeong‚ Eric Hamlett‚ Mihaela Mocanu and Oscar Alzate Summary Difference gel electrophoresis (DIGE) is most often used to assess relative changes in the expression levels of individual proteins in multiple complex samples‚ and this information is valuable in making inferences about relative protein activity. However‚ a protein‟s activity is not
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Why are sensorial materials an essential part of the prepared environment and how does the sensorial materials assist the child in his explorations of the world? Undeniably sensorial materials play a prominent‚ positive and profound influence to help the child in his exploration of the world around. In the early nineteenth century‚ the British romantic poets also could visualize the significance and importance of sensuous sensibility of five senses. Dr. Montessori contributed in putting her ideas
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VIETNAM NATIONAL UNIVERSITY – HOCHIMINH CITY INTERNATIONAL UNIVERSITY School of Biotechnology EXPRESSION OF PROTEIN ANTIGEN HIV -1 P24 IN TRANSGENIC TOBACCO PLANTS A thesis submitted to The School of Biotechnology‚ International University In partial fulfillment of the requirements for the degree of B.S. in Biotechnology Student name: Nguyen Ngoc Tu Anh – BT050036 Supervisor: Dr. Nguyen Thi Thanh
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Testing for reducing sugars‚ non-reducing sugars‚ starch‚ lipids and proteins in unknown substances: Our aim: was to find out which substances from our five samples have reducing sugars present to determine what they may be for example; they could be monosacchrides or disaccharides. This was carried out by using Benedict’s test. To find out which substances from our five samples were non-reducing sugars‚ since some disaccharides are reducing sugars and this would be carried out by using Benedict’s
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- ECTOTHERMS are organisms that have a limited ability to control their body temperature. Their cellular activities generate little heat. Their body temperatures rise and fall with ambient temperature changes. Most organisms are ectotherms. Examples are plants‚ all invertebrates‚ fish‚ amphibians and reptiles - ENDOTHERMS are organisms whose metabolism generates enough heat to maintain an internal temperature independent of the ambient temperature. Examples are birds and mammals - EXTENSION (Not
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Introduction Methionine and cysteine are both sulphur containing amino acids. Most proteins will contain one‚ or both of them at some point in the polypeptide chain. As such‚ many amino acids contain sulphur in some form‚ which is required in small amounts in the mammalian diet. Methionine has a thioether side chain‚ and cysteine’s contains a thiol group. These side chains exist as free thiols inside the cell‚ and are oxidised causing them to pair up and form disulphide bonds in an extracellular
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Protein synthesis occurs in two steps : Transcription and Translation. Transcription is the process of creating an mRNA copy of a DNA template; the mRNA is then translated into protein. The Messenger RNA (mRNA) contains the genetic information is copied from DNA during transcription . During translation‚ ribosomes synthesize the proteins using the mRNA copy produced during transcription. Proteins are complex molecules that each has a very unique shape‚ structure and function. The shape of the
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The Hair Follicle Stem Cell Niche and the Function of the Rac1 protein within the niche. Abstract: An adult stem cell’s niche is responsible for the preservation of the stem cell’s undifferentiated state through either intrinsic or extrinsic expression of specific proteins (Scadden‚ 2006). Situated in the bulge of the hair follicle‚ hair follicle stem cells (HFSC) are quiescent in nature and are responsible for not only its self renewal but also in the production of rapidly proliferating cells
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Do Fats Digest More Slowly Than Carbohydrates and Proteins? The foods we eat are classified into six elementary groups: carbohydrates‚ fats‚ proteins‚ vitamins‚ minerals‚ and water. These foods are broken down into various nutrients during digestion. The time taken for the body to extract nutrients and the complexity of the digestion process depend on the molecular nature of the food eaten. Fat molecules seem more complex than the others so its digestion often takes a longer time. Types of Fat Fats
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to effect. During the years different policies and different considerations have been developed to understand‚ fight or reduce criminality. This essay will attempt to identify whether environmental considerations to design out criminality actually assist in reducing criminal activity or simply end up being a short-term solution to a bigger problem that never goes away. The model of Crime Prevention through Environmental Design (CPTED) will be examined and its practical application in neighbourhoods
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