Human Embryonic Kidney(HEK) 293 cells were transiently transfected with pEGFP-N1(Clontech‚ Palo Alto‚ CA‚ encoding enhanced GFP as an indicator of transfection) and pEGFP-LeIF plasmids cloned at previous studies(Ghafarifar and et al‚ in press) by Calcium phosphate and non- tranfected cells was used as negative control. briefly‚ Hek-293 cells were grown at 37°C in Dulbecco’s Modified Eagle Medium (DMEM‚ Gibco) supplemented with 10% Fetal Bovine Serum (FBS‚ Gibco) and 5% CO2. Log-phase cells were employed
Premium DNA Gene Immune system
Finance University of Dhaka. 31 October 2011 Supervisor’s Certificate This is to certify that the internship report on “An Overview of Credit Performance of Prime Bank Limited” in the bona-fide record is done by Md. Asaduzzaman Khan‚ ID.No:13-010 as a partial fulfillment of the requirement of BBA Degree from the Department of finance‚ University of Dhaka. The report has been prepared under my guidance and is a record of the bona-fide work carried out successfully. …………………………………
Premium Debt Asset Balance sheet
Name: Summer Assignment: Report Topic: WishKids Foundation Introduction Last year‚ WishKids Foundation uses $500‚000 grant from the government‚ to help almost 10‚000 children in need. It also takes family picnic as a measure of the short of fundraising. More than $65‚000 raised‚ thorough donations and the sale of food or some merchandise‚ is to be sent to disadvantaged children and their families on holiday. The problem This year‚ the government will not allocate the grant‚ which has funded
Premium Finance Fundraising Economics
REPORT INTO THE ETHICAL CLAIMS OF THE BODY SHOP | | Student Name : Mitul Kabutarwala Student ID : Kamic2b Word Count : | | Contents Summary 1 Introduction 2 Claim 1 Community fair trade 2 Claim 2 misleading the public 2 Claim 3 Against Animal testing 3 Conclusion 3 Summary Over the past 10 years or so‚ since the time Body shop was taken over by L’Oreal many reports‚ articles have been written on the issue and all most every article or report conclude it
Premium The Body Shop L'Oréal
Social issue: Human trafficking… In this report I will be writing about human trafficking in Wales‚ Europe and other parts of the world. Human trafficking is an illegal activity. This involves selling‚ buying and trading of people. They are transported away from their families and communities and are forced to work against their own will. People are trafficked both between countries and within the borders of a state. It is also known as modern slavery and a form of sexual exploitation; where women’s
Premium Human trafficking Prostitution Slavery
Human Resources Management Assessment Approach January 2013 Paul Marsden‚ Liverpool Associates in Tropical Health (LATH) Margaret Caffrey‚ Liverpool Associates in Tropical Health (LATH) Jim McCaffery‚ Training Resources Group‚ Inc. (TRG) Human Resources Management Assessment Approach Human Resources Management Assessment Approach TABLE OF CONTENTS Introduction 1 Background 1 Purpose 2 Applying the Assessment Approach 3 Users 3 Time frame 3 Methodologies
Premium Healthcare Health care Medicine
UNIVERSITY P.M.B 2002 AGO-IWOYE OGUN-STATE A TECHNICAL REPORT ON STUDENTS INDUSTRAIL WORKING EXPERIENCE SCHEME (SIWES) COMMENCED FROM NOV. 1ST 2012 – FEB. 28TH 2013 UNDERTAKEN AT NO 33‚ SAKA TINUBU STREET VICTORIA ISLAND‚ LAGOS. PRSENTED BY ADEYEMI OLUWAFUNMILAYO IFEOLUWAPO MATRIC NUMBER: 09085849 DEPARTMENT: COMPUTER SCIENCE LEVEL: 400 CERTIFICATION This is to certify that this report was clearly written by ADEYEMI OLUWAFUNMILAYO
Premium IP address Ethernet Computer network
The term CV is short for the Latin phrase ’curriculum vitae ’ which simply means ’direction of life ’. In today ’s working world‚ a CV is a personalised document used by job seekers to provide a concise insight and overview into who they are‚ their educational and employment history‚ skills‚ interests‚ achievments and contact details. Along with a cover letter‚ CVs are one of the first things seen of a candidate by a prospective employer as you are required to supply them when applying for a job
Premium Accounts receivable Accounting software Accounts payable
Human development is very important in terms of living conditions in different countries. The statement "any society committed to improving the lives of its people must also be committed to full and equal rights for allEis true. The UN considers three factors to calculate human development in a country. These factors cover many aspects of a country‚ including social development in a country. Income‚ education‚ and healthy living are considered to be the most important factors in human development
Premium Human Development Index Developed country Life expectancy
Human Trafficking is one of the fastest growing crime industries in the world. Human trafficking is a phrase that is used to describe the various ways in which a person “obtains or holds another person in compelled service” (U.S. Department of State‚ 2011‚ p. 9). There are several categories of human trafficking‚ such as forced labor‚ sex trafficking‚ and bond or debt labor. Human trafficking can affect adults and children‚ with the trafficking in children for sex being particularly bad. Human trafficking
Free Human trafficking