Respiratory Acidosis and Alkalosis Activity 1: Normal Breathing 1. At 20 seconds‚ pH = 7.41 2. At 40 seconds‚ pH = 7.38 3. At 60 seconds‚ pH = 7.39 4. Did the pH level of the blood change at all during normal breathing? If so‚ how? Yes it did. It went down and then back up a little bit. 5. Was the pH level always within the “normal” range for the human body? yes 6. Did the PCO2 level change during the course of normal breathing? If so‚ how? No it did not Activity 2a: Hyperventilation
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speeds the breakdown of hydrogen peroxide. Catalase is present in every plant and animal organ. This is useful because hydrogen peroxide is harmful to cells. Temperature effects the enzyme effectiveness. Increase in temperature causes molecules to move faster. Too high of a temperature will cause an enzyme to denature. The enzyme loses its original shape and the active sight will no longer cause an reaction. Materials wax pencil 3 clean test tubes catalase hydrogen peroxide ruler Procedure First
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serves as a biological catalyst (Denniston‚ 2007). Metabolic reactions happen with in cells. Enzymes are proteins that are used to speed up these reactions without being consumed by them (Mader‚ 2010). Catalase is a catalyst that digests potent hydrogen peroxide and converts it into H2O and O (Campbell Reese‚ 2008). The environment plays an important role in the reaction that enzymes have. In this experiment‚ enzymes were exposed to changes in temperature‚ pH‚ and concentration. Introduction
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the pH (concentration of hydrogen ions)‚ remain within a fairly narrow range. Extreme variations in pH and temperature denature the enzyme by altering its chemical structure‚ thus adversely affecting the chemical reaction. As even the slightest change in the protein’s structure will change the enzyme’s shape enough to prevent the formation of the enzyme-substrate complex‚ the reaction will then be unable to take place. The enzyme‚ catalase‚ reacts with hydrogen peroxide in order to yield water and
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write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a household blender •potatoes (3 medium size potatoes‚ that are peeled and quartered) •tap water •8 test tubes •8 transfer pipets •sugar packets •salt •a small saucepan •a refrigerator with a freezer •stove •4 clear plastic cups •tongs (to remove test tubes from boiling water) •4 coffee cups •cheesecloth to filter the potato
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how temperature affects enzyme activity. Hypothesis: I believe that the room temperature activity will produce the most activity. Materials: 3% hydrogen peroxide‚ a household blender‚ potatoes (3 medium size potatoes‚ that are peeled and quartered)‚ tap water‚ 8 test tubes‚ 8 transfer pipets‚ sugar packets‚ salt‚ a small saucepan‚ a refrigerator with a freezer‚ stove‚ 4 clear plastic cups‚ tongs (to remove test tubes from boiling water)‚ 4 coffee cups‚ cheesecloth to filter the potato extract
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different sources: Spinacia oleracea (Spinach)‚ Brassica oleracea (Broccoli)‚ Solanum tuberosum (Russet Potato)‚ Malus domestica (Apple)‚ and Allium cepa (Onion). The five different catalases from the sources will all be used to catalyze Hydrogen peroxide (H2O2). When hydrogen
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When liver was added to hydrogen peroxide is caused and instantaneous reaction time. This is due to the enzyme catalase. If the enzyme catalase was not present in the human body‚ hydrogen peroxide‚ which is highly toxic to cells‚ would attack the unsaturated fatty acid compounds in the membrane of lipids.2 This would cause substantial damage to the membrane structure. Since catalase utilizes hydrogen peroxide as both an electron acceptor and donor it oxidizes hydrogen peroxide isolating oxygen while
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11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity studies reflect
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in almost every living organism that is exposed to oxygen. For this experiment‚ potatoes were used because they were previously known to have significant catalase activity. The potato catalase was mixed with hydrogen peroxide because the catalase helps to breakdown the hydrogen peroxide which helps determine how long a paper disk takes to float to the surface‚ which in return provides the time of the complete reaction. Since various assays were mostly used in combination with the potato extract
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