on the reaction rate of an enzyme IB biology Internal Assessment 3/23/12 Research Question: Effect of changes in substrate concentration amount on the reaction rate of an enzyme Introduction: In this experiment‚ the substrate is hydrogen peroxide. The purpose of this investigation is to find out the relationship between the substrate concentration and the rate of reaction. Substrates are molecules that are acted upon by enzymes. For instance‚ amylase‚ an enzyme found in saliva‚ helps
Premium
Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
Premium Oxygen Hydrogen peroxide Enzyme
of the most common enzymes. It is found in living organisms and is used to break down hydrogen peroxide. This must happen because hydrogen peroxide is considered toxic to cells in the body. However‚ when catalase is used it breaks it down into hydrogen and oxygen‚ which is much safer to have in the body. A substrate is the material that is being broken down. In this case‚ the substrate would be hydrogen peroxide. The catalase‚ located in the liver‚ is being used to break down the substrate. Problem
Premium Chemical reaction Hydrogen peroxide Water
source eg. Radishes/celery Hydrogen peroxide Range of buffer solutions pH paper Washing up liquid Disposable gloves The apparatus for this experiment lends itself to being pre-prepared in a ‘box of equipment’ – see Section 1. Advance preparation Prepare buffer solutions. Obtain fresh celery. Advance chemical preparation (a) Hydrogen peroxide (Prepares 250 ml of 1M/’12 vol’ solution) Wearing gloves‚ measure out 29 ml of ‘100 vol’ hydrogen peroxide into a measuring cylinder
Free PH Buffer solution
I. Experiment 1 A. Description of Experiment: Three plastic beakers were used for this experiment. In one‚ a 20 mL of 1% hydrogen peroxide solution was made. In order to get this‚ the stock solution‚ which was 3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay
Premium Hydrogen peroxide Catalase Oxygen
Purpose: Cells produce toxic wastes‚ in this experiment hydrogen peroxide‚ and without some sort of molecule to break it down the cell will die‚ along with the organism itself. However with the aid of an enzyme‚ catalase‚ hydrogen peroxide is able to be broken down into an intermediate and thereafter harmless substances water and oxygen. The goal of this lab is to measure the reaction rate of this process in different substances such as a liver‚ a vegetable‚ and breast tissue. By using variables
Premium Hydrogen peroxide Enzyme Chemical reaction
techniques to investigate the effect of different concentrations of the enzyme catalase on the rate of breakdown of hydrogen peroxide. Background information Catalase is an enzyme which is found in all living organisms. This enzyme catalases the decomposition of hydrogen peroxide into water and oxygen. Cells continually produce a poisonous by-product of metabolising‚ called hydrogen peroxide. This is very damaging to cells and is broken down as soon as it is formed in the cytoplasm into water and oxygen
Premium Enzyme Catalase Metabolism
Francis Biology 183 Abstract This experiment was performed to determine the resultant effect of temperature change on the reaction between the enzyme catalase and hydrogen peroxide. This experiment was performed by measuring and comparing the amount of oxygen bubbles produced and the absorbance of the catalase and hydrogen peroxide solution over time at room temperature‚ 2°C‚ 50°C‚ and 60°C. The overall result of this experiment proved that this reaction works best at room temperature‚ slows down
Premium Chemical reaction Enzyme Chemistry
good rule of thumb is to remember that enzyme names end in “-ase”. This will help in identifying enzymes in further readings. Generally enzymes are catalysts. Hydrogen peroxide is a toxic chemical that is produced in many organisms during metabolism. Organisms must get rid of this toxin to survive. One reaction turns the hydrogen peroxide into water and oxygen. The enzyme that helps with this reaction is called catalase. This is found in both plants and animals. In this lab we will use potatoes as
Premium Enzyme Chemical reaction Metabolism
as the potato‚ hydrogen peroxide is broken down to release water and oxygen. Mixtures were allotted time to react‚ with those have a longer time resulting in more substrate being able to be acted upon in correlation. The sulfuric acid was added after the allotted time and acted as an inhibitor. The inhibitor left a mixture of the products and original hydrogen peroxide. Our titrant of potassium permanganate (KMNO4) shows through its purple color the remnants of the hydrogen peroxide‚ which we were
Premium Oxygen Hydrogen peroxide Enzyme