what affects the rate of reaction. We used Hydrogen peroxide to test the rate of reaction‚ with the temperature of this being our variable that we changed. Hydrogen peroxide is a clear‚ colourless liquid which has various amounts of uses within the laboratory‚ industrial purposes and even in our households. It is mainly used for cleaning products and hair dye but is also used for paper making. Hydrogen peroxide is made up of two oxygen atoms and two hydrogen atoms. The chemical formula for this is H₂O₂
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The 0% concentration of Hydrogen Peroxide was used as our control. The 3% concentration of Hydrogen Peroxide was provided so all that was needed to do was prepare the 0%‚ 1%‚ and 2% concentrations which was done by using the equation C₁V₁ = C₂V₂. Other materials consisted of pH 7 buffer‚ guaiacol which was used as our
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Place each measuring cylinder in each water-filled beaker. 5. Pour 81 ml of hydrogen peroxide into an empty beaker and label it ‘Hydrogen Peroxide’ to clarify its contents. Get a dripper bottle of detergent and transfer 9 ml of hydrogen peroxide from the beaker using a dropper and place the amount in a small measuring tube. Put two equal drops (as close to equal as possible) of detergent along with the 9ml of hydrogen peroxide in the small measuring tube in each measuring
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the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which slowly decreases in the course of an hour. This decrease in
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the rate of catalase activity was discovered in this lab. The assay system‚ in which a filter paper disc was dipped into the enzyme and submerged using a stirring rod in a test tube filled with 20mL of hydrogen peroxide‚ was used to test several enzyme factors. As the saturation of hydrogen peroxide increased the rate of reaction increased as well. When the enzyme concentration increased the rate of catalase activity increased too. When catalase was subjected to an increase of temperature changes
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Classifying Reactions Safety Reminder: Wear safety glasses and use ammonia in a well-ventilated area. Day 1 Materials: Part I: steel wool tweezers vinegar small jar or drinking glass water small bowl Part II: hydrogen peroxide small pieces of raw potato (yeast or beef liver may be substituted for the potato) small bowl Procedures: Part I: Reaction between iron and oxygen 1. Break off a small piece of steel wool and soak it in vinegar for at least one minute. Use tweezers to push the steel
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Bubbles after the addition of hydrogen peroxide indicate a positive catalase test. The method for the catalase test was to incubate the plate of the bacterium at 35C for 24 to 48 hours. Afterwards‚ drop hydrogen peroxide on the colonies and observe them for bubbles. Bubbles were formed after the addition of hydrogen peroxide‚ which indicated that it did produce enzymes that can break down hydrogen peroxide to water and oxygen. The next step according to the previous
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Factors affecting Enzyme Activity: The effect of pH on enzyme activity Background Knowledge: An enzyme is a biological catalyst – which speeds up the reaction rate‚ without itself getting altered. Enzymes are proteins with long polypeptide chains that are folded up into three – dimensional shapes. An enzyme acts on a substrate to convert the substrate into a product useful for the organism.The active site is a special region on the surface of the enzyme where the substrate binds to the enzyme.
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1/2 cup of baking soda 1/4 cup of hydrogen peroxide 1/4 cup of warm water Optional: 3 teaspoon of glycerin 3 teaspoon of xylitol 1 cup of cold water 1 cup of hot water 1/4 cup of water Steps 1. 1 Pour a half of a cup (110g) of baking soda into a mixing bowl. Baking soda has a natural cleansing ability and can even be found in some commercial toothpastes. It’s non-toxic and will help polish your teeth. Some recipes call for table salt‚ in which case you should mix three
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Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The
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