minimize the effects of inhibitory compounds during sulphuric acid (H2SO4) dilution. The treatment of biomass with H2SO4 (1.5%) at 100°C for 30 minutes exhibited optimum results. During enzymatic hydrolysis 16.52 mg/mL glucose was obtained by using 1 mL of enzymatic load at 50oC after 72 hours of hydrolysis. The Glucose thereafter converted to 5.21g/L and 3.69 g/L of ethanol during fermentation process by using Sacchromyces cervesiae and Fusarium oxysporum respectively. Introduction A variety of
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enzymes and which results indicate non-production of the given enzyme by placing a “+” or “-“in the appropriate squares. A. Starch Hydrolysis Note: the color of the medium immediately surrounding the colonies after addition of iodine on PDA plate. Organism Result + or - Appearance/Observations Bacillus cereus Escherichia coli B. Gelatin Hydrolysis (gelatinase production) Note: whether the gelatin deep remains solid or has been liquefied Organism Result + or - Appearance/Observations
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Microbiology 20 Laboratory Final Exam Guidelines The laboratory exam will be given on Tuesday December 4th (sections 0375 and 3252) or Thursday December 6th (section 3253) and consist of an open book portion given in CMS 105 followed by a practical portion (closed book) to be given in the lab (CMS 002). You will not need a Scantron. For the open book test‚ be sure to bring any resources you may need including your lab manual‚ lab notebook‚ safety guidelines and handouts. For the practical portion
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Abstract In an environment isolation procedure‚ experiments under categories‚ such as‚ morphology‚ physiology‚ antibacterial susceptibility‚ selective media‚ and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to
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of organisms oxidize glucose for energy: In animals excess glucose is converted to a polymer called glycogen. Disaccharides On hydrolysis give two molecules of monosaccharides E.g Sucrose (Cane sugar) Lactose (milk sugar) Maltose (malt sugar) Polysaccharides Starch‚ cellulose‚ glycogen On the hydrolysis of each of them‚ they yields large number of monosaccharides. Monosaccharides also known as simple sugars classified by 1. the number
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Anthony Young Partner: Nick Dale TA: Alehkya 6/8/15 Isolation of Trimyristin from Nutmeg and Preparation of Myristic Acid from Trimyristin by Hydrolysis. Introduction In this experiment‚ trimyristin will be isolated from the nutmeg by extraction‚ and then used to prepare myristic acid by hydrolysis. The isolation of a substance from a natural product is a common technique used nowadays. Usually‚ the isolation is a complex procedure and requires a lot of steps and preparation. For this experiment
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Introduction: According to chemists‚ hydrolysis is defined as the breaking of a compound involving a reaction with a water molecule. The goal of this experiment is to three-dimensionally show how disaccharides are transformed into monosaccharides using an acid-catalyzed hydrolysis reaction. For the purpose of this lab‚ it is quite useful to fully understand the following terms: optical rotation and carbohydrate. Optical rotation is measured by a polarimeter‚ which measures the polarization of light
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Light-Orange Aldohexoses E. IODINE TEST FOR POLYSACCHARIDES Compound Color Polysaccharides (yes/no) Water Orange No Glucose Orange No Fructose Orange No Sucrose Orange No Lactose Orange No Maltose Orange No Starch Black Yes Glycogen Black Yes F. HYDROLYSIS OF DI- AND
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Objectives After studying this Unit‚ you will be able to • define the biomolecules like carbohydrates‚ proteins and nucleic acids; • classify carbohydrates‚ proteins‚ nucleic acids and vitamins on the basis of their structures; • explain the difference between DNA and RNA; • appreciate the role of biomolecules in biosystem. Biomolecules “It is the harmonious and synchronous progress of chemical reactions in body which leads to life”. 14 Unit A living system grows‚ sustains and reproduces
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Acetone‚ MEK and Methyl Isobutyl Ketone‚ May 1972 Report No. 77 ACETONE‚ METHYL ETHYL KETONE AND METHYL ISOBUTYL KETONE by SHIGEYOSHI TAKAOKA May 1972 A private report by the PROCESS STANFORD ECONOMICS RESEARCH INSTITUTE PROGRAM I MENLO I PARK‚ CALIFORNIA Acetone‚ MEK and Methyl Isobutyl Ketone‚ May 1972 CONTENTS a 1 INTRODUCTION. . . . . . . . . . . . . . . . . . . . . . . . 1 2 SUMMARY 3 3 4 5 . . . . . . . . . . . . . . . . . . . . . . . . . . Acetone .............
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