The reaction between alpha-amino acid and ninhydrin involved in the development of color are described by the following five mechanistic steps: alpha-amino acid + ninhydrin ---> reduced ninhydrin + alpha-amino acid + H2O alpha-amino acid + H2O ---> alpha-keto acid +NH3 alpha-keto acid + NH3 ---> aldehyde + CO2 Step (1) is an oxidative deamination reaction that removes two hydrogen from the alpha-amino acid to yield an alpha-imino acid. Simultaneously‚ the original ninhydrin is reduced
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effects Acids and Bases Acknowledgement Some material have been sourced from the following websites and books Reference • http://facstaff.gpc.edu/~matteya/organicppt/Ch2.ppt • http://atom.chem.wwu.edu/dept/facstaff/pavia/351pavia.html • www.fccj.us/chem1212/powerpoint/Ch17_Lewis_B.ppt • CH102 Course book • Organic Chemistry 7th Edition by John McMurry 2 Acids & Base Definitions Definition #3 – Lewis • Lewis acid - a substance that accepts an electron pair • All BrØnsted-Lowry acids are also
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patel (rmp932) – Post-Lab 5 Acid/Base Titration – lyon – (51730) This print-out should have 25 questions. Multiple-choice questions may continue on the next column or page – find all choices before answering. 001 10.0 points Answer the question to at least six significant figures. A solution of sodium hydroxide is standardized against potassium hydrogen phthalate (KHP‚ formula weight 204.2 g/mol). From the following data‚ calculate the molarity of the NaOH solution: mass of KHP 1.404 g; buret reading
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The Role of the Citric Acid Cycle The Citric Acid Cycle is a series of enzyme-catalysed reactions that take place in the mitochondrial matrix of all aerobic organisms. It involves the oxidation of the acetyl group of acetyl CoA to two molecules of carbon dioxide. Each cycle produces one molecule of ATP by substrate-level phosphorylation‚ and reduces three molecules of NAD and one molecule of FAD for use in Oxidative Phosphorylation. The cycle is preceded by Glycolysis‚ which also occurs in anaerobic
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Acid-Base extraction theory states that when an acid reacts with an organic base it results in a salt that is water-soluble and a neutral molecule that is insoluble. The addition of an acid to a mixture containing acids and bases will result in the acid remaining unchanged and the base reacting to form a salt. The results from the experiment were fairly consistent when separating the strong acid‚ weak acid‚ or the base. In part one of the experiment‚ you had to separate the strong acid. When added
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A. Title – Acid-Base Extraction with Separatory Funnel B. Introduction – The main objective of the experiment was the extract an acid‚ a base‚ and a neutral compound from a 2:1:1 compound of benzoic acid‚ p-nitroaniline‚ and azobenzene. In theory‚ an extraction technique based on phase distribution should allow two immiscible solvents to separate with a portion of the solute distributed into each. Using a separatory funnel‚ acids were extracted with bases (NaOH)‚ and bases were extracted
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Determination of Ka for a Weak Acid Introduction In the experiment preformed the objective is to titrate a weak acid with a strong base. In a titration of a weak acid with a strong base the titrant is the strong base and the analyte is a weak acid. The reaction that will occur is the direct transfer of protons from the weak acid to the hydroxide ion. The data gathered will be represented on the titration curve‚ a graph of the volume of titrant being the strong base plotted against the pH .The
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TITLE : * Analysis of an unknown acetic acid solution OBJECTIVES : * To prepare the sodium hydroxide solution‚ NaOH * To standardise the base against potassium hydrogen phthalate * To analyse the unknown acetic acid RESULTS : A. Preparation of the sodium hydroxide solution Volume of NaOH taken from the stock solution = 3.33 mL B. Standardisation of the base against potassium hydrogen phthalate | 1 | 2 | 3 | Weight KHP | 1.0000 | 1.0004 | 1.0006 | Final volume
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Title: ACID BASE TITRATION. Objectives: 1. To determine the concentration of acid using titration. 2. Skills of titration techniques. Apparatus: 1. 250 volumetric flask 2. 10mL measuring cylinder 3. 25mL pipette 4. 50mL burette 5. 250mL beaker 6. 150mL conical flask 7. Retord stand 8. White tile 9. Stopwatch 10. Pipette bulb Chemicals: 1. HCl solution 2. 0.1M NaOH solution 3. H2SO4 solution 4. Distilled water 5. phenolphthalein
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I. Introduction The purpose of this experiment is to determine the pH values of acids‚ bases‚ and buffers of distilled water and 10.0 buffer using measured concentrations of Sodium hydroxide (NaOH) and/or Hydrochloric acid (HCl). Acid is a compound typically having a bitter taste and capable of nullifying alkalis and releases hydrogen ion when added to a solution‚ or containing an atom that can accept a pair of electrons from a base (McKinley‚ Dean O’Loughlin‚ & Stouter Bidle‚ 2016). Bases are water-soluble
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