inhibiting molecules used clinically to help treat conditions such as glaucoma‚ myasthenia gravis and Alzheimer ’s disease (Scapin‚ 2006; Liston et al.‚ 2004). In this particular experiment‚ we are attempting to calculate the rate of the reaction (hydrolysis) of different substrates by enzymes acetylcholinesterase and butyrylcholinesterase‚ in the presence of various inhibitors (anticholinesterases). Acetylcholinesterase (or erythrocyte cholinesterase) is mainly found in red blood cells‚ neural synapse
Premium Acetylcholine Enzyme
identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate reduction‚ etc. Biochemical testing is very important in the medical field when a patient is infected with an unknown microbe. Such testing can identify the microbe(s) and types of medications effective in treating the infection. It is
Premium Bacteria Microbiology Gram staining
Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The
Premium Bacteria Microbiology Microorganism
minimize the effects of inhibitory compounds during sulphuric acid (H2SO4) dilution. The treatment of biomass with H2SO4 (1.5%) at 100°C for 30 minutes exhibited optimum results. During enzymatic hydrolysis 16.52 mg/mL glucose was obtained by using 1 mL of enzymatic load at 50oC after 72 hours of hydrolysis. The Glucose thereafter converted to 5.21g/L and 3.69 g/L of ethanol during fermentation process by using Sacchromyces cervesiae and Fusarium oxysporum respectively. Introduction A variety of
Premium Cellulose Lignin Yeast
enzymes and which results indicate non-production of the given enzyme by placing a “+” or “-“in the appropriate squares. A. Starch Hydrolysis Note: the color of the medium immediately surrounding the colonies after addition of iodine on PDA plate. Organism Result + or - Appearance/Observations Bacillus cereus Escherichia coli B. Gelatin Hydrolysis (gelatinase production) Note: whether the gelatin deep remains solid or has been liquefied Organism Result + or - Appearance/Observations
Premium Enzyme Starch
Microbiology 20 Laboratory Final Exam Guidelines The laboratory exam will be given on Tuesday December 4th (sections 0375 and 3252) or Thursday December 6th (section 3253) and consist of an open book portion given in CMS 105 followed by a practical portion (closed book) to be given in the lab (CMS 002). You will not need a Scantron. For the open book test‚ be sure to bring any resources you may need including your lab manual‚ lab notebook‚ safety guidelines and handouts. For the practical portion
Premium Bacteria Microbiology Metabolism
Abstract In an environment isolation procedure‚ experiments under categories‚ such as‚ morphology‚ physiology‚ antibacterial susceptibility‚ selective media‚ and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to
Free Bacteria
of organisms oxidize glucose for energy: In animals excess glucose is converted to a polymer called glycogen. Disaccharides On hydrolysis give two molecules of monosaccharides E.g Sucrose (Cane sugar) Lactose (milk sugar) Maltose (malt sugar) Polysaccharides Starch‚ cellulose‚ glycogen On the hydrolysis of each of them‚ they yields large number of monosaccharides. Monosaccharides also known as simple sugars classified by 1. the number
Free Glucose Carbohydrate Starch
Anthony Young Partner: Nick Dale TA: Alehkya 6/8/15 Isolation of Trimyristin from Nutmeg and Preparation of Myristic Acid from Trimyristin by Hydrolysis. Introduction In this experiment‚ trimyristin will be isolated from the nutmeg by extraction‚ and then used to prepare myristic acid by hydrolysis. The isolation of a substance from a natural product is a common technique used nowadays. Usually‚ the isolation is a complex procedure and requires a lot of steps and preparation. For this experiment
Premium Chemistry Distillation Mixture
Introduction: According to chemists‚ hydrolysis is defined as the breaking of a compound involving a reaction with a water molecule. The goal of this experiment is to three-dimensionally show how disaccharides are transformed into monosaccharides using an acid-catalyzed hydrolysis reaction. For the purpose of this lab‚ it is quite useful to fully understand the following terms: optical rotation and carbohydrate. Optical rotation is measured by a polarimeter‚ which measures the polarization of light
Premium Chemical reaction Enzyme Chemistry