6 M HCl medium using tri-n-butyl phosphate‚ selectively separates Indium from accompanying elements in different type of geological samples. Acid hydrolysis of Nb/Ta samples separates Indium from major matrix elements like Nb and Ta and the remaining elements do not influence the selective extraction and preconcentration of In and its subsequent determination by ICP-AES or flame AAS. The silica rich geological samples are decomposed by HF-H2SO4-HCl treatment followed by dissolution in 6M HCl before
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of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart‚ I proceeded to inoculate a Starch plate. In week 2‚ I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative due to the dark color. Next‚ I inoculated Simmons Citrate Agar slants. By week 3 I was able to analyze the Citrate Agar‚ which tested negative for citrate utilization. With each
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galactose‚ maltose‚ fructose‚ sucrose‚ lactose‚ glycogen and starch. | Carbohydrates‚ in acidic condition‚ form furfural‚ which reacts to Anthrone which gives a bluish-green-coloured complex. | Blue colour formation | MOORE’S | Concentrated NaOH | Mix 1ml of 5% glucose and 0.5ml of concentrated NaOH. Boil and note the change of color and odor produced. Do the same with 5% galactose‚ maltose‚ fructose‚ lactose‚ sucrose‚ glycogen and starch. | When a solution of reducing sugar is heated with an alkali
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Ye Tao BISC220-13155 The Effect of Temperature on the Digestion of Starch by Activity of Enzyme α-Amylase: Observation of Rate of Starch Disappearance through Iodine Test Introduction An enzyme is a type of protein that‚ through its own structure including hydrogen bonds‚ acts like a biological catalyst and is able to accelerate the biochemical reaction rate by lowering the activation energy of the whole process‚ without which cells could hardly practice any physiological functions within human
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Guide Questions Experiment 1 Calibration of the calorimeter: 1. Give the net ionic thermochemical equation of the reaction used to calibrate the calorimeter. a. Is the reaction endothermic or exothermic? b. Which is the limiting reactant? c. How much (in moles) limiting reactant was used? d. How much heat was generated (or absorbed) by the reaction? 2. Relate the sign of the ΔT to the ΔH of the reaction used for calibration. 3. What is the heat capacity of the calorimeter? Relate
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10. Cysewski G.R.‚ Wilke C.R.‚ (1976). Utilization of cellulosic materials through enzymatic hydrolysis and fermentation of hydrolysate to ethanol and single cell protein. Biotechnol. Bioeng.18: 1297– 1313. 11. Dale B.E.‚ Moreira M.J.‚ 1982. A freeze explosion technique for increasing cellulose hydrolysis. Biotechnol. Bioeng. Symp. 12‚ 31-43. 12. Taherzadeh MJ‚ Karimi K (2007). "Acid-based hydrolysis processes for ethanol from lignocellulosic materials: A review" 13 18. Wheals‚ A. E.; Basoo
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reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat is a form of energy and would increase the reaction and speed up the reaction time. It would also cause the starch to hydrolyze before cooler temperatures
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Kinetics of an SN1 reaction: the effect of solvent on rate Object The purpose of this experiment is to determine the rate of hydrolysis in acetone/water (50/50 v/v and 60/40 v/v). Background and Theory An SN1 reaction of tert-butyl chloride takes place in two steps. First‚ the Alkyl Halide will leave the molecule. In this step the bond is breaking‚ which takes a longer amount of time‚ so it will determine the rate of the reaction. As a result‚ it forms a tertiary carbocation‚ since this
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functions of some of these biomolecules are discussed in this Unit. 14.1 Carbohydrates Carbohydrates are primarily produced by plants and form a very large group of naturally occurring organic compounds. Some common examples are cane sugar‚ glucose‚ starch‚ etc. Most of them have a general formula‚ Cx(H2O)y‚ and were considered as hydrates of carbon from where the name carbohydrate was derived. For example‚ the molecular formula of glucose (C6H12O6) fits into this general formula‚ C6(H2O)6. But all
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each culture Bacillus subtilis‚ Pseudomonal aeruginosa‚ and Escherichia coli‚ to each of the separate sections. The plate was incubated until the next lab period‚ where we then flooded the plate with Iodine and were able to observe and record starch Hydrolysis. (Table 1) * In order to test carbohydrate catabolism under anaerobic conditions we tested cultures of Pseudomonas aerugenosa‚ Escherichia coli‚ and Alcaligenes faecalis in tubes containing a glucose medium. After inoculating each tube
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