IODINE TEST FOR STARCH The Iodine test is used to test for the presence of starch. Iodine solution — iodine dissolved in an aqueous solution of potassium iodide — reacts with the starch producing a purple black color. The colour can be detected visually with concentrations of iodine as low as 0.00002M at 20°C. However the intensity of the colour decreases with increasing temperature and with the presence of water-miscible‚ organic solvents such as ethanol. Also the test cannot be done at very low
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The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
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A-2Time Blue Color Fades | A-3Time for Starch Hydrolysis | 1 | 1 | 9:07 am | 9:42 am | 35 minutes | 2 | 5 | 9:07 am | 9:37 am | 30 minutes | 3 | 10 | 9:08 am | 9:33 am | 25 minutes | 4 | 15 | 9:08 am | 9:28 am | 20 minutes | 5 | 30 | 9:09 am | 9: 24 am | 15 minutes | Figure 1: Enzyme Activity (Time for Starch Hydrolysis) vs. Enzyme Concentration B. Effect of pH pH | B-1Starting Time | Time Blue Color Fades | B-2Time for starch Hydrolysis | 3 | 9:17 am | 9:47 am | 30 minutes
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glucose‚ fructose‚ maltose‚ lactose‚ sucrose and starch. There was six tests that were carried out to help identify them‚ these were: Iodine Test‚ Solubility in Water‚ Benedict’s test‚ Acid Hydrolysis‚ Barfoed test and Diastix test. Aim The aim of the experiment which was carried out was to identify the unknown carbohydrates by subjecting them to a series of biochemical tests. The tests were as follows: Iodine‚ Solubility‚ Benedict’s‚ Acid Hydrolysis‚ Diastix and Barfoed test. Methods There
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QUESTIONS 1. What are other sources of glycogen? Of starch? a. Other sources of glycogen- Aside from the liver‚ it can also be found in skeletal muscles (for energy during strenuous exercise). It also occurs in tissues including adipose tissues‚ heart muscles‚ kidneys and the brain. It is also found in yeast‚ bacteria‚ fungi‚ molds and algae‚ oysters‚ and shellfish. b. Other sources of starch- Foods that are high in starch include breads‚ grains‚ cereals‚ pasta‚ rice‚ potatoes‚ peas
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reducing and non-reducing sugars. Glucose is monosaccharide while maltose‚ lactose and sucrose are disaccharides of carbohydrates. Monosaccharaides are the monomers which make up all other carbohydrates and cannot be broken into smaller molecules by hydrolysis. Disaccharides are formed from the condensation reaction between two monosaccharaides and are linked by glycoside bond. All monosaccharaides and some disaccharides such as maltose and lactose are reducing sugars due to the presence of aldehyde or
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|Observations of the Reaction| A.|NaHCO3 + HCl| H12|White and bubbled slightly and settled down to smaller smaller bubbles| B.|HCl + BTB| H11|Golden yellow; looked darker under the black paper| C.|NH3 + BTB|H10 |Dark blue| D.|HCl + blue dye| H9|Greenish color| E.|Blue dye + NaOCl| H8|Blue and lighter than NH3+BTB solution color| | with the 1 drop of HCl| H8|Turned blue green in color| F.|NaOCl + KI| |See back page| | with 1 drop of starch| || G.|KI + Pb(NO3)2| H6|Bright yellow |
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empirical formula: deoxysugars‚ aminosugars Carbohydrate - polyhydroxy aldehyde‚ ketones. General characteristics Most carbohydrates are found naturally in bound form rather than as simple sugars Polysaccharides (starch‚ cellulose‚ inulin‚ gums) Glycoproteins and proteoglycans (hormones‚ blood group substances‚ antibodies) Glycolipids (cerebrosides‚ gangliosides) Glycosides Nucleic acids Classification of carbohydrates Monosaccharides Trioses‚ tetroses
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Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and
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most useful substances for many living organisms: Carbohydrates. Carbohydrates are used for numerous processes in organisms from acting in important roles of cellular recognition in the human body to storing chemical energy in plants in the form or starch. The roles of carbohydrates in the human body alone are numerous; providing energy for working muscles‚ providing energy for the regulation of blood glucose‚ preventing proteins being used for energy‚ breaking down fatty acids and playing a part
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