Abstract The experiment is to identify the guilty suspect that is present at the crime scene by comparing with the DNA samples. Polymerase Chain Reaction(PCR) is used to amplify the small amount of Deoxyribonucleic Acid (DNA) for forensic or genetic studies‚ which require necessary product and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using
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A2 Chemistry Coursework Section 1 Aims: I aim to find out the order of reaction with respect to [H2O2] and [2I-]. I aim to find out the activation enthalpy of the reaction by finding the rate of reaction at different temperatures using the Arrhenius Equation. The experiment will go as follows: Into a conical flask put 15cm3 of distilled water and add 2cm3 of [X]moldm-3 potassium iodide (KI) solution and 1cm3 of 2moldm-3 sulphuric acid. Then add to this 2.5cm3 of 5vol (0.42moldm-3) hydrogen
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Aim: This experiment will aim to show how the amount of substrate affects the rate of the reaction. Hypothesis: In this experiment I think the amount of substrate will simply increase the reaction. As I increase the surface area of the potato the gas given off from the reaction will increase. Therefore to sum things up‚ my hypothesis is when the Independent variable increases so will the dependant variable. Independent variable: - Surface area of potato Dependant variable: - Gas Controlled
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Limiting Reactant and Percent Yield in a Precipitate Reaction (SMG 6D) AP Chemistry One example of a double replacement (metathesis) reaction is the mixing of two solutions resulting in the formation of a precipitate. In solution chemistry‚ the term precipitate is used to describe a solid that forms when a positive ion (cation) and a negative ion (anion) are strongly attracted to one another. In this experiment‚ a precipitation reaction will be studied. Stoichiometry will then be used
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find the order of a reaction Introduction When peroxodisulfate (VI) ions and iodide ions react together in solution they form sulfate (VI) ions and iodide. This reaction is shown below: S2O82-aq+ 2I-aq SO42-aq+ I2(aq) The reactants and the sulfate (VI) ions are colourless however the Iodine is a yellow/brown colour. This allows you to measure the progress of the reaction through the colour change when the iodine is produced. In order to determine the order of the reaction we need to measure
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The Polymerase Chain Reaction and Determination of Alu Population Freqencies Porshia Gibbs April 8‚ 2010 Genetics Laboratory Abstract Cheek cells were extrapolated and used in PCR amplification and electrophoresis of the amplified samples to determine the presence or absence of the dimorphic Alu sequence in a class population. A bioinformatic allele server was then employed to calculate genotypic and allelic frequencies of the Alu element in the class population. The Hardy-Weinberg equation
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Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended in the lysis solution. They were rinsed in a buffer before going through centrifugation. - Polymerase Chain Reaction (PCR): The isolation done‚ the Drosophila cDNA located in the plasmid DNA was amplified. The PCR was used to determine the size of the cDNA inserted into the plasmid DNA. One tube (A) contained the plasmid DNA A with the PCR master mix‚ the other
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PLAN Introduction: After having built up knowledge about the kinetics of reactions I decided to do an investigation in this area. I was initially introduced to this particular reaction1 in EP6.4 and then in AA2.1. I was interested in using this reaction as a means of potentially supporting and quantifying some of the theories that I have studied along with also perhaps extending on them. Aim: Using a clock reaction I shall: Investigate the effect of concentration for each reactant and use
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equation of the reaction of hydrogen peroxide and iodide ions. This will be achieved by using an iodine clock method and colorimetric analysis. 2. Draw a graph of rate against concentration for each reactant (Hydrogen peroxide‚ potassium iodide and H+ ions). 3. Finding the order for each reactant 4. Finding the rate-determining step. 5. Proposing a mechanism for the reaction. 6. Using Arrhenius’ equation to find the activation enthalpy. Background The basic reaction for this can be illustrated
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times 6‚ 7‚ 8 hours on reaction time (seconds) in humans?” concluded that the group sleeping six hours had an average reaction time of 0.54 seconds‚ the group sleeping seven hours had an average reaction time of 0.60 seconds‚ and the group sleeping eight hours had an average reaction time of 0.45 seconds. The hypothesis was somewhat supported with the group sleeping eight hours having the highest reaction time‚ however‚ the group sleeping seven hours hours had a slower reaction time than those sleeping
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