Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing the most amount
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* Proteins were originally thought to be the molecule of heredity because they were more complex than DNA‚ were very present‚ and DNA was only found on chromosomes * * S type → dead mouse * R type → healthy mouse * S type (heat killed) → healthy mouse→ no S cells isolated from mouse * S type (heat killed) plus R → dead mouse * S type → dead mouse * R type → healthy mouse * S type (heat killed) → healthy mouse→ no S cells
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humans and can be used as a way to distinguish between males and females. Gender determination of a human has important applications in forensic science‚ prenatal diagnosis‚ etc. This can be done by Polymerase Chain Reaction (PCR) followed by gel electrophoresis. The presence of two bands of DNA indicates that the sample is from a male while the presence of one band of DNA indicates that the sample is from a female (sasaki and shimokawa‚ 1995). In this experiment‚ Chelex was used to extract nucleic
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We came up with certain hypothesis that are based on our predictions: “Users experience an “illusory correlation” when they are asked to rank the generic brands.” Users tend to think that the generic brand would be less tasty even though in reality that’s often not the case. However because it is a generic brand they have prejudices against it. We expect participants to create an illusory correlation between the brand‚ its quality and the price. In our case
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The two visual systems hypothesis talks about the processing of the visual information in two different routes in the brain (van Polanen & Davare‚ 2015). This idea was first introduced in 1982 when Leslie Ungerleider and Mortimer Mishkin described experiments that distinguished two streams that served different functions(Melvyn A. Goodale‚ 1998). They used the method know as ablation or also called lesioning. Using both recordings from neurons and ablation‚ they found that properties of the ventral
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The “Descent from Teosinte” hypothesis helps define the difference between maize and teosinte. According to Iltis‚ corn is cultivated teosinte‚ they do not differ in genetic or vegetative features (Goodman‚ 1988). Human select qualities in corn that produce more food‚ larger quantity‚ and efficient yield. Maize and teosinte have the same amount of chromosomes and similar structure due to common descent. At the DNA level‚ teosinte and maize have the same number of chromosomes and almost the same arrangement
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microscopes simulated electrophoresis and much more. MATERIALS: The materials we used : 1) The Lab packet 2) Foam cups 3) Chromatography paper 4) Pen or Pencil 5) Microscope slides for species x ‚y ‚z ‚and the Botana Curus 6) Microtip dropper 7) Plant extract 8) Microscope 9) H2O Procedures:
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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Lab: investigating hooked law with springs Purpose: to find spring constants of different springs using the slope of a graph of change in heights vs. the weight force. Also‚ to be able to understand how spring constants change when you add springs in a series or paralle Pre lab predictions: We predicted that the graph of gravitational force (mg) as a function of stretch (delta x) would look like Data: Spring #1: y = 8.2941x + 0.0685 This table represents the different distances that
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