The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
Premium DNA Gene Molecular biology
The purpose of lab number thirteen was to examine the response of the daphnia to a chemical stimulant. Also examined was how human resting heart rates differed from the daphnia’s. Stimulants are any substances that raise the levels of activity in the body. Stimulants could be a number of things such as physical or chemical. Physical stimulus usually deal with something that affects the five senses such as sight‚ hearing these could be attributed to loud noises or too much light going into your eye
Premium Drug addiction Addiction Heroin
AuthorDate ____________ Lab Section # ___________________________ Lab Partner BIOL 1208 Writing Assignment 1 Results Worksheet These are to be written individually even if you worked in a group. Lab Topic: ________________________________________________ Introduction: What was your null hypothesis? be specific A change in temperature in the reaction of catechol and catecholase will not change the absorbance of reaction over time. What was your alternative hypothesis? be specific As the environment
Premium Null hypothesis Chemical reaction Hypothesis
years. Roderick also has a sister‚ Madeline‚ who he loves as a sister and a partner. Some people may draw the conclusion that the Ushers were insane and others may say that the Usher estate is haunted and influenced by the supernatural. One common hypothesis is that Madeline was influenced by supernatural blood sucking beings also known as vampires. There are many key details throughout the story that strongly back this conjecture. Some of these details include Madeline’s actions during the story and
Premium The Fall of the House of Usher Edgar Allan Poe English-language films
3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
Premium DNA Protein Molecular biology
Criteria 6 8 10 Title Your lab report has a Title that is directly related to the lab experiment/ exercise. Your group or class must be able to come up with your own title You must make your own title based on scientific theory Aim You have clearly stated your aim Your aim is relevant and informative You must make your own aim based on scientific theory Background background information relevant to the lab experiment/ exercise You must be able to link your aim to the background The
Premium Theory Scientific method Experiment
Lab Report: Scientific Method Your Name: Alyssa Melincoff Purpose of this Lab What is the goal of this lab? What question is it trying to answer‚ or what problem is it trying to explain? To find out how table salt affects the boiling temperature of water. Hypothesis After reading the lab instructions - but before starting the lab - record your best “educated guess” about what will happen in the experiment. What hypothesis can you generate about the effect of adding salt to water? Adding
Premium Water Scientific method Temperature
Egg Lab Write Up The purpose of this lab was to depicked the many properties of diffusion such as isotonic‚ hypotnonic‚ and hypertonic‚ so that students could have a clear visual example to go by for future refrences. We also did this to learn about selective permeability and osmosis. Our original hypothesis stated that the vinegar would react with the calcium in the shell to create CO2 bubbles. Note I said the original hypothesis as this experiment took coarse over a four day period‚ in which
Premium Osmosis Hypothesis Observation
Title: Penny Lab Experiment By -Objectives To determine how soap affects the surface tension of water. -Hypothesis In this experiment the hypothesis was that soap was going to affect the surface tension by weakening the bonds and allowing less water on the penny. -Materials * Penny * Beaker x2 (One containing soapy water the other containing pure tap) * Paper towels * Lab review sheet * Pencil * Pipette -Procedure First we picked who would drop the water onto
Free Scientific method Hypothesis Theory
as shown in the ClassPak. The statement in parentheses refers to the Alternative Hypothesis for each. 5. On average IU students go out 2.5 nights or more per week. (The alternative hypothesis is that IU students go out less than 2.5 times per week.) Ho: μNights >= 2.5 Ha: μNights < 2.5 6. On average IU students drink exactly 5 1/2 drinks in an average night out. (The alternative hypothesis is that IU students do not drink 5 1/2 drinks per night out.) Ho: μDrinks
Premium Null hypothesis Statistical hypothesis testing Hypothesis