dye mix‚ and DNA marker pBR322/BstNI in a freezer (approximately –20°C). All other materials may be stored at room temperature (approximately 25°C). Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance with prudent laboratory safety precautions and under the supervision of a person familiar
Premium DNA Polymerase chain reaction Molecular biology
Phase Change Lab Ha Min Noh Ms. D Chemistry February 6‚ 2016 Question How does time relate to the change in temperature of H2O as it changes phases from ice to water to gas? Hypothesis If the time which H2O stays on the hot plate increases‚ then the temperature of H2O will increase and the phases from ice to water to gas will change because the heat is a source of energy to change the physical states. Background Ice is a solid form of H2O and water is a liquid form of H2O. The gas form of H2O
Premium Thermodynamics Temperature Gas
Chemistry 12 - Lab 19A Flow Chart (Investigating Chemical Equilibrium) PART 1: Put on safety goggles and lab apron Obtain 2 (Empty‚ Clean‚ AND DRY) 250mL Erlenmeyer flasks. Add approx. 100mL of DISTILLED water and 1mL of thymol blue solution to each flask Record the colour of this solution in note book FIRST FLASK: add a single drop of 0.1M HCl. Swirl the contents of the flask and continue drop-by-drop addition until a definite colour change is observed. SECOND FLASK: will serve as
Premium Red Color Chemical reaction
subject: Serum protein electrophoresis. Report No.: 1. Serum Protein Electrophoresis (SPEP) Introduction: The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. And its uses an electrical field to separate the proteins in the blood serum into groups of similar size‚ shape‚ and charge. And here we’ll use gel electrophoresis which indicate that blood serum is placed on special paper treated with agarose gel and exposed to an
Premium Molecular biology Gel electrophoresis Protein
OUTLINE What is PCR and Gel Electrophoresis? • Polymerase chain reaction (PCR) is a technique which is used to amplify the number of copies of a specific region of DNA‚ in order to produce enough DNA to be adequately tested. This technique can be used to identify with a very high-probability‚ disease-causing viruses and/or bacteria‚ a deceased person‚ or a criminal suspect. • Gel electrophoresis is a widely used technique for separating electrically charged molecules. It is a central technique
Premium DNA
Hypothesis: The bigger the area‚ the larger species it can support. Aim: To see how the size of an area affects the relationship between species diversity. Diversity is a range of different elements. Habitat size determines diversity relationship(Edition of Nature‚ March 28 2002). In the research of specie diversity ‚ it was found out that more productivity was done when two different types of species were in one area. It was also said that diversity goes down but as it goes down the productivity
Premium Biodiversity Ecology Species
mays. My hypothesis is that the environment that has the light all the time will show the best germination. The reason why I am doing this experiment is to find which lighting is the best for the germination of Z. mays. With the information from the experiment‚ I can
Premium Seed Fruit Germination
Restriction Enzymes and Electrophoresis Bhumik Patel Phillips 1/16/11 Restriction enzymes are tools in DNA research that can cut DNA into exactly needed pieces. Certain cuts can be rough‚ while others can be clean. Certain cuts can have an organized pattern to have a staggered cut. Other cuts will leave complementary bases with them. Electrophoresis allows the manipulation of DNA to separate and organize those parts. Electrophoresis is the substrate electric movement of the separation
Premium DNA Molecular biology Enzyme
steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page 7 to page 10) Results: (I) Spectrophotometric Determination of DNA A260 = 3.923 A280 = 3.923 Conversion factor
Premium Bacteria Molecular biology Protein
Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
Premium Amino acid Electric charge Acid