EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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The word ‘Hypothesis is derived from a Greek word‚ which means ‘to suppose’. It is usually considered as the principal instrument in research. For a researcher it is a formal question that he or she intends to resolve. In this way a hypothesis may be defined as a proposition or a supposition. The main function of hypothesis is to guide the collection and processing of materials and direct the research. Hypothesis is a tentative conclusion. It is facts based theory. A research scholar will analyze
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enzymes and Agarose Gel Electrophoresis. The objective of this lab was to match a criminals DNA to a crime scene using restriction enzymes EcoRI and Pstl with Agarose gel electrophoresis. Restriction enzymes cut DNA at a specific base pair site recognized by the enzyme‚ which then turns one single strand of DNA into many fragmented strands of DNA. EcoRI recognizes and cuts the palindromic base pair sequence GATTC while Pstl recognizes and cuts the palindromic base pair sequence CTGCAG. Agarose gel electrophoreses
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In conclusion‚ the purpose of the lab Bird Adaptations was to act as a bird using different tools as a beak‚ and try to collect as many items as you can at each station in 15 seconds. Overall‚ the data collected throughout this lab supported each of my hypothesis. The data shows that certain “beaks” worked better than the other at collecting food. For example‚ the data shows that for the Seeds Station the tweezers worked best with an average of 9.3 pieces of food‚ the straw worked best for the Nectar
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step 2: The hypothesis Answer the following questions: * What is a hypothesis? * How is a hypothesis different from a theory in science? * Describe an example of how you may use the scientific method in your daily life and state a good hypothesis for that example. * Which hypothesis did you first pick in the "Recognizing a good hypothesis" activity in the scientific method tutorial? Why? If any‚ which mistake(s) did you make when picking a hypothesis? A hypothesis is a specific
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The team of forensic anthropologists ran a gel electrophoresis with DNA from Skeleton 3 and two missing persons‚ Julia Ly and Teresa Chen to help in DNA identification. This process would allow restriction enzymes to cut by a specific restriction site and run through the gel‚ where the DNA fragments would move from the negative side to the positive side of the gel due to the negative charge of the phosphate group in DNA. The smaller the DNA fragments‚ the further they move down the gel. As mentioned
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Helicopter Lab November‚ 2012 Kevin‚ Lucy‚ Charlotte Objective or Question What is the most effective helicopter design for landing the slowest? Hypothesis The most effective helicopter design that allows itself land the slowest would be a cylindrical cone with many small‚ rectangular wings attached to its base. Equipment & Materials Materials: Equipment: Index cards Scissors Scotch tape Timer Paperclips Measuring tape Meter stick Procedure: 1). Fold an index
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1. How does the process of gel electrophoresis separate DNA fragments? It uses an electric current to separate different-sized molecules in a porous‚ sponge-like matrix. 2. What is the purpose of the agarose gel? It is used to separate DNA molecules that range in different lengths. 3. What is the purpose of adding blue “tracking” dye to the DNA samples? The blue tracking dye is added to help load the samples easily and helps able to see the DNA moving through the gel. 4. Explain why DNA has
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The reason for this experiment was to figure out which solution would prevent the apples from browning the most and to determine if pH has an effect on the prevention of browning of the apples. My hypothesis stated that white grape juice would prevent the apples from browning the most. This hypothesis was refuted‚ lemon was actually the solution that prevented the browning of the apples the most. Before the apples were dipped in the solution‚ the average rating of the apples on a scale of 0 to 5
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1. The central purpose of this paper is to describe all of the methods of how PCR was developed and the results of the experiments involving extraction and amplification of DNA (Mullis et. al. 1986). 2. PCR has the ability to isolate specific DNA sequences with the use of primers. This is done by denaturing the DNA (at 95o C) so it is able to anneal to the primers that specify a fragment to be amplified (Mullis et. at. 1986). These primes anneal to a specific sequence of DNA in order to amplify
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