difficile and why is it relevant to us? A.“Clostridium difficile (C. difficile) is a bacterium that may develop due to the prolonged use of antibiotics during healthcare treatments.” 1 B. “Clostridium difficile is an obligate anaerobe or microaerophilic‚ gram-positive‚ spore- forming‚ rod-shaped bacillus.” 2 II. What are the signs and symptoms of Clostridium difficile infection (CDI)? A. C. difficile can cause symptoms ranging from diarrhea to life-threatening inflammation of the colon (pseudomembranous
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Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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(2013) reported a method for isolation of E. coli O157 from meat sample. Twenty five grams of meat sample was blended with 225 ml of sterile modified tryptone soya broth containing vancomycin (40 μg/ml) followed by incubation at 37 °C for 18 h. Enriched culture was plated onto sorbitol MacConkey agar supplemented with cefixime and potassium
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The spot inoculation method was followed for antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚
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sample was taken from a door handle to the George Lynn Cross Hall‚ which is touched by hundreds of students entering the building every day. The most important exercises performed include gram staining‚ which
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CHAPTER I INTRODUCTION 1. ENTEROCOCCUS FAECALIS AND STAPHYLOCOCCUS AUREUS OUTLINE The Human indigenous micro flora consists of opportunistic pathogens along with other nonpathogenic bacterial strains. Two eloquent members of the category of gram- positive bacteria‚ Enterococcus faecalis and Staphylococcus aureus belong to this category. Being a facultative anaerobe‚ aero tolerant anaerobe respectively‚ both of these organisms inhabited the human body for centuries. Ability to thrive in extreme condition
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_____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the colonies formed: Yeasts: Moist‚ creamy bacteria-like‚ opaque‚ or pasty colonies on media. They reproduce by budding. (when they start budding‚ they cause infections) Molds (filamentous fungi): Fluffy cottony‚ woolly or powdery colonies on medium. They reproduce by sporulation. 2. Describe or
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BACTERIA Period: 4 Characteristics: 3 major shapes Cocci Basilli Spirilla 3 major components Mesosomes flagella Plasmids Growing Up: Bacteria can obtain energy through phototrophs(sunlight)‚ lithotrophs(inorganic compounds)‚ and organotrophs(organic compounds) Marriage/Reproduction Binary Fission: The process by which all bacteria reproduce. It results in the separation of a single cell into two. Transformation: genetic alteration
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unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative. I also used the microscope to identify
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