bilimbi) leaves and fruit extract as an antibacterial agent that is more or equally effective than the commercialized one and at the same time‚ available in our environment. Ten Replicates on ten Petri dishes were prepared for each bacteria. Pure cultures of the three bacteria were spread on the replicates. After extracting the needed substances from the kamias leaves and fruit extract‚ the researchers were able to begin the experimentation. Six treatments were made in the set-up-Treatment A as 100% water
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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antibiotic that works on gram negative bacteria. Gram-negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol whereas Gram-positive bacteria are those that are stained dark blue or violet by Gram staining. The bacteria we used were E-coli that are a gram negative bacteria and the only antibiotic out of Penicillin and Streptomycin that will work is Streptomycin. Penicillin is also effective antibiotic but it will only work on bacteria that Is gram positive because
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12th December‚ 2014 MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria‚ which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of identification
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| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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Bacterial Diseases of Fish by Robert B. Moeller Jr.‚ DVM California Animal Health and Food Safety Laboratory System University of California Aeromonas hydrophila (Bacterial Hemorrhagic Septicemia) 1. Gram negative motile rods 2. Effects many freshwater species and usually is associated with stress and overcrowding. 3. The clinical signs and lesions are variable. The most common finding is hemorrhage in skin‚ fins‚ oral cavity and muscles with superficial ulceration of the
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential
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instability‚ usually bactericidal. It hinders or kills bacteria. Ceftriaxone sodium is a broad-spectrum bactericidal cephalosporin antibiotic. Ceftriaxone is active in vitro against a wide range of Gram-positive and Gram-negative organisms‚ which include β-lactamase producing strains. Ceftriaxone is indicated in the treatment of the following infections either before the infecting organism has been identified or when known to be caused by bacteria of established sensitivity. Fig. 1.2 Chemical Formula
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