Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on the basis
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semester‚ I have learned multiple techniques for identifying bacteria. Learning how to gram stain‚ use specific media such as MacConkey agar‚ and test antibiotics to see which antibiotic would react best against a specific organism. All these techniques helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive or negative. I created
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(4) uses of energy in biological processes (B); active transport; muscle contraction; nerve transmission; synthesis; translocation; nitrogen fixation; receptors ENERGY TRANSFERS WHICH TAKE PLACE INSIDE LIVING ORGANISMS Living organisms including all plants and animals require energy for their cellular processes. In biological processes‚ the immediate energy source is often in the form adenosine triphosphate (ATP). The nucleotide ATP maintains both catabolic and anabolic reactions. An example of
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Unicellular organism From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search "Single-celled" redirects here. For prison cell assignment‚ see Single-celling. Valonia ventricosa is among the largest unicellular species. A unicellular organism‚ also known as a single-celled organism‚ is an organism that consists of only one cell‚ unlike a multicellular organism that consists of multiple cells. Historically the simple single celled organisms have sometimes been referred to as monads
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Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli Proponents: John Rovic P. Ngo Lester T. Almarez Trisha Mae N. Reyes August 2013 In Partial Fullfillment of the Requirements in Research II-B Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli A Science Research Project Presented to The Faculty of Science and Technology Mr.June Hayden Sinson Research Adviser
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Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive
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Helicobacter pylori | Description | Helicobacter pylori is a motile‚ curved‚ spiral‚ or straight‚ slightly plump‚ gram-negative rod. It is believed to be a major cause of gastritis and peptic ulcers although it resides solely on the gastric mucosa and does not enter cells. Microscopic description for the family Campylocbacteraceae | Shape: Spiral ShapedStaining Reaction (Gram): NegativeUnique Characteristic: Spiral Shaped with Flagellum Single/Clusters: ClusterSpore Formation: NoDimension:
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Gaskin September 10‚ 2014 Minimal Organism “A minimal genome is generally defined as the smallest set of genes that allows for replication of the organism in a particular environment (Cho‚ M.‚ 1999). The creation of these minimal genomes can be helpful and harmful at the same time. There are many opinions out there about the subject‚ I will share some of those and my position on the subject. First let me just start with my personal position on minimal genomes. I personally feel that the use of them
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difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are
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When I received my slant culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started
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