– Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the reaction tubes using a new micropipette tip for each transfer of DNA and enzyme (refer to figure
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the beginning of the lab. Post-lab questions for Topic 5 – Enzymes Name: Date: Group: T W R Formation and Detection of Benzoquinone Table 1. Formation and Detection of Benzoquinone: Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate‚ enzyme and product of the enzymatic reaction? 2- What is the purpose of tubes 2B and 2C? Enzyme Specificity Table 2 – Specificity of Cathecol oxidase for different
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Name______________________________________________ IB Guide to Writing Lab Reports Standard and Higher Level Chemistry 2010-2011 Table of Contents page 1 Explanations‚ Clarifications‚ and Handy Hints page 2 - 13 IB Laboratory Evaluation Rubric page 14 - 15 Formal Lab Report Format page 16 Error Analysis Types of Experimental Errors page 17 Error Analysis: Some Key Ideas page 18 Precision and Accuracy in Measurements A Tale of Four Cylinders Assessment of Errors and Uncertainties in IB Lab Reports
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Part B: Practical Report The Effect of Temperature on Enzyme Activity Aim: To investigate how temperature effects the enzyme catalase. Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water‚ when compared to cold water. Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner. Introduction: An enzyme is a protein‚ which speeds up a specific chemical reaction without altering
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Enzymes Abstract: The following 2 labs experimented the more enzymes and substrates added to the concentration will effect the reaction rate. Our second lab‚ we tested enzyme and substrate concentrations to determine the increase of temperature and inhibitor. The enzyme source used in both labs was peroxide‚ guaiacol is used as a substrate for peroxide. We used Guaiacol‚ turnip extract‚ peroxide and distilled water for enzyme and substrate concentration. In the second lab we used the same substances
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DIGESTIVE ENZYMES AND THEIR FUNCTIONS Mouth -the oral cavity or the mouth contains salivary glands that secrete a wide range of enzymes to aid the first step of metabolism of food. The salivary gland secretes the enzyme lysozyme which has an antibacterial action. The enzymes secreted by the mouth mainly provide protection against bacteria. Betaine helps to maintain a fluid balance inside the mouth. The lists of digestive enzymes secreted by the oral cavity are mentioned in the table. Enzymes | Function
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of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules
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The Effect of Enzymes on Apple Juice Production Biology Period 6 Background Info The purpose of this experiment was to determine which enzyme or the combination of the two enzymes makes the most apple juice from applesauce. We did his by‚ mixing 10 drops of an enzyme (cellulose/pectinase/both) into a few tablespoons of applesauce. We then left the rest to nature‚ and watched the liquid funnel out for 10 minutes. Lastly‚ we recorded our data. The cell wall is a complicated
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RUNNING HEAD: Understanding Enzymes Understanding Enzymes Amanda Chung Blanche Ely High School Abstract The researcher conducted this experiment in order to investigate how various factors effected enzymes. Those factors were the concentration of the enzyme‚ the temperature it had to work in‚ and the pH level of the solutions as well. All three were hypothesized to speed up the rate at which the enzyme acted. A series of several tests were carried out to find the answer to each problem
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The Effects of Varying pH on Enzyme Activity A lab was conducted to test different pH balances on an enzyme. Introduction: Enzymes are protein catalysts that speed up a chemical reaction without being consumed in the process. Enzymes are three-dimensional structures that consist of one or more polypeptide chains. The polypeptide chains form an active site (where a substrate will fit into). Enzyme molecules are folded into a very specific shape held together by the different forces of attraction
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