………………………………………………………………………………..9 Properties of Enzymes and Competitive Inhibitors. Abstract: Properties of enzymes were found in this experiment and some other factors‚ which affect enzyme activity. Enzymes are catalyst; they catalyze very specific reactions. Results relating to the active site of specific enzymes played a big role while performing this experiment. The purpose of this experiment was to fin how inhibitors affect enzyme’s activity by competing for the active
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Abstract The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor
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How can we recognize when we have made progress in the search for knowledge? Consider two contrasting areas of knowledge. The search for knowledge is a constantly ongoing process. So how are we to know if we have made any progress? Progress is development‚ advancement‚ and moving forward. In this case it is the progress in the search for knowledge in an area of knowing. Within the areas of knowing the strongest contradiction is between human science and natural science. Human sciences
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Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because we assume the more substrate an enzyme gets
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Exploring Enzymes - Ground-Up Tissue Activity Abstract Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined
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IB Biology Potato Lab Table 1: Trial Number | Concentration of Sucrose Solution (M) ±0.2 ml | Initial Mass of Potato Core Slice(g) ±0.1 | Final Mass of Potato Core Slices (g) ±0.1 | 1 | 0.0 | 7.7 | 9.3 | 2 | | 6.0 | 8.1 | 3 | | 6.2 | 7.4 | 4 | | 10.2 | 13.2 | 5 | | 8.7 | 10.3 | 6 | | 4.9 | 6.0 | 7 | | 9.2 | 10.4 | 1 | 0.2 | 5.8 | 6.0 | 2 | | 11.6 | 12.1 | 3 | | 2.5 | 3.1 | 1 | 0.4 | 14.4 | 13.9 | 2 | | 2.6 | 2.8 | 3 | | 8 | 6.5 | 1 | 0.6 | 7.3 | 5.3 | 2
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Lab Ex#8: "Enzymes: Catalysts of Life" INTRODUCTION Enzymes are protein organelles where chemical reactions take place to generate energy within our cells. Without the energy produced from the cell enzyme activity‚ we would not possess the catalyst activity necessary for energy to produce movement. Each enzyme performs a specific function within our bodies. Those functions performed can be significantly altered with the introduction of variables outside their environment. Variables‚ such as temperature
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The purpose of this experiment is to study how enzyme activity is affected by environmental conditions. Researchers tested the level of potato extract enzyme activity with 1-11 pH‚ varying temperature‚ catechol solution‚ hydroquinone solution‚ and different measurements of catechol. In Figure 1A and 1B‚ pH levels were tested with potato extract to see how pH would affect the amount of Benzoquinone is formed in the potato. Although it was hypothesized that enzymes would form Benzoquinone better in
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IB Interesting Facts 1968‚ IB‚ standing for the International Baccalaureate‚ has been founded as a non-profit educational organization at the International School of Geneva. It was created by teachers at the school‚ in addition to help from teachers from all over the world. Today‚ the International Baccalaureate has grown into a university preparatory program that has been divided‚ in most schools‚ into three programs for students as young as 3 years old to students 19 years old. • It was first
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Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life’s processes would not come so easily. Enzymes are basically proteins‚ which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down
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