I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
Premium Molecular biology DNA
Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
Premium Enzyme Enzyme inhibitor Catalysis
Banana and Papaya Fruit Peelings Extract as an Alternative Culture Medium for Fungi Submitted by: Maricel P. Clores Catherine A. Bernardo Rodora Lafiguera Julius Jorge Suarez Scope and Delimitation This study is focused on how to produce ointment out of banana and papaya fruit peelings extract to help avoiding fungal infections. It must be done within a laboratory. The place must be conducive for only a matter of years. This study builds upon on how to lessen fungal
Premium Fungus
IB HISTORY SYLLABUS & COURSE OUTLINE 11th & 12th Grades – Standard and Higher Level OVERVIEW Our ignorance of history causes us to slander our own times. -‐ Gustave Flaubert The study of History is far more than the memorization of names and dates from the past. Although the focus
Premium Soviet Union History
2013-2014 Standard Level IB Business and Management Internal Assessment Will the acquisition of Tumblr by Yahoo significantly help this former “internet pioneer regain traction with younger internet users”? Word Count: 1438 Candidate Name: Phoebe‚ Leung Ching Lam Candidate Number: 003069-037 Class: F6.CassiaContents Page 1. Introduction The purpose of this essay is to investigate whether the acquisition of Tumblr by Yahoo will significantly help the former Internet pioneer regain
Premium Income statement Google Balance sheet
Year 11 IB Biology IA Report 2011 5/31/2011 Enzyme Activity Experiment Introduction 3 AIM 3 Materials 3 Method 4 Result 5 Table 1: 5 Table 2: 5 Graph 1 6 Discussion 6 Conclusion 8 Bibliography 9 Appendix 1 (Test tube 3- 45 degrees) 9 Appendix 2 (Test tube 1- 5 degrees) 9 Appendix 3 (Test tube 2- 20 degrees) 9 Introduction Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8.3 phenolphthalein will
Premium
Hypothesis: the higher the concentration of hydrogen peroxide the faster the reaction will occur and the more foam will be produced. Aim: The aim of this investigation is to determine how the concentration of hydrogen peroxide effects the intensity of the reaction. Variables: Independent- The concentration of hydrogen peroxide. Dependant - The intensity of the reaction. Constants – The temperature‚ the amount of liver‚ pH level‚ test time‚ Materials: Solutions of hydrogen peroxide varying
Premium Hydrogen peroxide Liver Oxygen
LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables
Premium Enzyme PH Blood
IB Mathematics SL Year 1 Welcome to IB Mathematics. This two-year course is designed for students who have a strong foundation in basic mathematical concepts. The topics covered in this course include: * Algebra * Functions * Equations * Circular functions * Trigonometry * Vectors * Statistics * Probability * Calculus ------------------------------------------------- Resources: * Textbook: Mathematics SL 3rd edition. Haese Mathematics 2012 ISBN: 978-1-921972-08-9
Premium Assessment Homework help service Homework
Lab report nº4 The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which
Premium Hydrogen peroxide Pressure Atmospheric pressure