Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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Received July 18‚ 1997X A radiochemical enzyme assay for studying cyclooxygenase (COX)-catalyzed prostaglandin biosynthesis in vitro was optimized with respect to both COX-1 and COX-2 activity. The assay can be used to assess the relative selectivity of plant-derived inhibitors on COX-1 and COX-2. Assay conditions were optimized for both enzymes with respect to concentration of cofactors (l-epinephrine‚ reduced glutathione‚ and hematin)‚ activation time (enzyme and cofactors)‚ reaction time‚ and
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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The written assignment is based on one work in translation studied in part 1 of the IB course syllabus. For us‚ that means Medea‚ Blood Wedding‚ or Ghosts. Students produce an analytical‚ literary essay (1‚200-1‚500 words) plus a reflective statement (300-400 words) undertaken during the course and externally assessed by the IB. The process This foundation for this assignment has been completed already. Now we have reached the last part of the process‚ which is choosing a work‚ developing a
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at a particular temperature‚ a reaction mixture obeys the Law of Chemical Equilibrium‚ which imposes a condition on the concentrations of reactants and products. This condition is expressed in the equilibrium constant Kc for the reaction. In this experiment‚ I will study the equilibrium properties of the reaction between ethanoic acid‚ otherwise known as acetic acid (CH3COOH) and water (H2O): CH3COOH(aq) + H2O(l) CH3COO-(aq) + H3O+(aq) When solutions containing ethanoic acid and water are mixed
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Experiments: Doctors‚ Experiments‚ and Results Melissa Anjeanette Edwards POLYTECH High School of Kent County‚ Woodside‚ Delaware Abstract During World War II experiments were done on the prisoners of war in Nazi Germany. Doctors for these camps came in all shapes and sizes including former S.S. Troops‚ Women‚ and a variety of prisoner doctors. The experiments differed as much as the doctors themselves; however they stayed the same in one factor‚ medical curiosity become killing in atrocious
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Acids And BAses Acids And BAses 8.1 8.2 8.3 8.4 18.1 18.2 18.3 18.4 18.5 Theories of acids and bases Properties of acids and bases Strong and weak acids & bases The pH scale Calculations involving acids and bases (AHL) Buffer solutions (AHL) Salt hydrolysis (AHL) Acid-base titrations (AHL) Indicators (AHL) 8 8.1 THeORies OF Acids And BAses 8.1.1 Define acids and bases according to the Brønsted–Lowry and Lewis theories. 8.1.2 Deduce whether or not a species could act as a Brønsted–Lowry
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DISCUSSION: Bromelian added to Gelatin: Bromelian is an enzyme found in pineapples. When Bromelian is added to gelatin it breaks down the protein and does not allow the gelatin to solidify. There are several factors that can cause an enzyme to slow down or to completely stop reacting. For example‚ temperature and pH can effect enzyme activity. Canned pineapple juice and fresh pineapple juice were used to see how the enzyme would react differently. In fresh pineapple juice the Bromelian have would
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for this course is demanding. However‚ always remember that the goal of this course is to give you the best possible preparation for your college career – you may even earn college-level credit The purpose of this course is to prepare you for the IB exam and for college. Since your exam will be taken at the end of your senior year‚ it is critical that you save your assignments and notes in an organized format. You will need them to study for the exam. We will be creating study guides‚ note cards
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the okay to do the experiment‚ I grabbed the girls and went to work. The experiment I picked was the conservation concept‚ where the idea that merely changing the appearance of objects does not necessarily change other key properties. In other words‚ just because I put the same amount of water in a bigger case‚ doesn’t change how much water there is. I predict that both the girls would fail at conservation concept‚ and always
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