Transformation is the genetic alteration of a cell‚ resulting from the intake of exogenous genetic material through the surrounding cell membrane. The purpose of this lab was to determine transformation of bacteria by testing the effect of P Vib plasmid of E. coli MM294‚ and how the color of the E. coli bacteria changes. In this lab‚ two small test tubes were given calcium chloride‚ E. coli MM294‚ and one of the tubes also received the plasmid P Vib. The test tubes were then placed in ice‚ heat shocked
Premium
Transformation of Bacterial Cells with Plasmid DNA Introduction: Transformation refers to the process in which the cell integrates foreign DNA to its genetic code‚ meaning it takes the genes and incorporates them into the cell’s current DNA. Cells that can do this naturally‚ most commonly bacteria and archea‚ are known as competent. The bacteria E. coli do not have high transformation competence under normal conditions‚ but can be manipulated to produce better results using
Free Bacteria Antibiotic resistance Plasmid
agricultural sector in particular and rural economy in general must play an indispensable part in any strategy of economic development. China is one of the countries which put this into practice after the 1949 revolution when it embarked on its rural transformation. During the past 50 years‚ China’s leaders experimented with various measures to develop the country’s rural areas. In the pre 1978 period‚ these measures were usually implemented in the form of mass mobilization campaigns‚ associated with various
Premium People's Republic of China Agriculture Deng Xiaoping
1. The Transformation of Prospero In Shakespeare’s "The Tempest" the figure of Prospero‚ the former Duke of Milan‚ forms the key element of all actions and developments throughout the play. He incorporates absolute power over the other characters and generates the plot of the play almost uninfluenced. For an in-depth understanding of the play it is therefore indispensable to analyse whether or not he undertakes a transformation in character or behaviour and hence interrupts a straight development
Premium The Tempest Supernatural Religion
When reading books it is quite enthralling to observe how essential just one character can be. In To Kill a Mockingbird by Harper Lee‚ the character Jem is Scout’s (the main character’s) older brother. Jem is short for Jeremy‚ Jeremy Atticus Finch. Jem starts the book as a 10 year old and the story concludes when he’s 12. Jem was a very likeable character who had a great influence on the story‚ his change/progress was interesting to witness as a reader. Jem has several remarkable experiences
Premium Atticus Finch To Kill a Mockingbird Harper Lee
Upon reading The Scarlet Letter‚ there were distinct changes in the way Hester was perceived and how she perceived herself. The complexity of what Hester’s life has become derives from the letter which represents guilt‚ pain‚ and betrayal; the most prominent of the three being the guilt she had to undergo. This constant reminder of failure is enough to destroy a human along with the isolation and separation she lived with. In Nathaniel Hawthorne’s‚ The Scarlet Letter‚ Hester has had to survive with
Premium
“This is a story of how a Baggins had an adventure‚ and found himself doing and saying things altogether unexpected. He may have lost the neighbours’ respect‚ but he gained -- well‚ you will see whether he gained anything in the end” (Tolkien 2). The Hobbit by J.R.R Tolkien presents an unlikely hero‚ a hobbit named Bilbo Baggins. Gandalf‚ a wizard and old family friend‚ forces Bilbo out of his comfort zone onto a journey to recover the dwarves home and gold from the evil dragon‚ Smaug. Bilbo fulfills
Premium The Hobbit Bilbo Baggins The Lord of the Rings
great themes make great novels”. In the novel A Room With A View‚ by E.M. Forster‚ this definitely proclaims true. One of the major themes in this novel is transformation. In the novel A Room With A View‚ by E.M. Forster‚ transformation is apparent through personal discovery‚ falling in and out of love‚ and the shifting of alliances. Transformation is first shown in the novel through personal discovery‚ and an example of this is by Lucy accepting other social classes. In the beginning of the novel Lucy
Premium Fiction Sociology Literature
Glowing Transformations Abstract In this experiment‚ the idea is to become familiar with the transformation of cells. A well thought out procedure‚ involving a heat shock procedure‚ a good antibiotic‚ an inducer known as arabinose to show the newly expressed DNA by a visible fluorescent glow‚ and a stable control group is what contributes to this experiments thoroughness. It is predicted that the four agar plates will all yield different forms of growth‚ with different coloration and colony
Premium Cancer
Transformation Tuesday is every Tuesday in the student union at 8 it is a religious event for christians. At this event you will become stronger in your faith without knowing it‚ this is because they put together christian games. The purpose of these games can be who says the strongest prayer‚ or who knows the bible verse and so forth. My first time attending this event was on halloween this event made me feel at home not only because of the welcoming faces but‚ also because I am use to going
Premium Christianity Jesus God