Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
Premium Ultraviolet DNA Plasmid
The story of IBM in India is surrounded by several myths and folklore‚ particularly relating to its infamous exit in 1978. George Fernandes‚ who was industry minister in the Janata government‚ is often credited for throwing out IBM over foreign exchange rules‚ but the trouble for IBM had started way back in 1967. It had a good run of the Indian market for two decades after its entry into India in 1951‚ facilitated by Jawaharlal Nehru himself. See: IBM’s 100-year journey‚ from clocks‚ scales
Premium Computer IBM Jawaharlal Nehru
Transformation in The Metamorphosis When people are put in tough situations‚ they are forced to adapt to their surroundings. The Metamorphosis demonstrates a particular theme that shows how people change when they are faced with hardships and sufferings. In the novella‚ Gregor transforms into a bug because of the pressure that results from his very dependent family and his job. Gregor’s sister‚ Grete‚ and their father‚ Mr. Samsa‚ also change their behavior and attitude because of the distress Gregor’s
Premium The Metamorphosis Franz Kafka
The Transformation of BP Chapter 5-4 | Eric | Summer | Helen | Lidya | Mandy | Content • Introducing BP • Transforming BP • BP Superior performance through Knowledge Sharing • BP Building Cooperate Reputation Through facing the Public Responsibility • Conclusion and Future Consideration Introducing BP BP at a Glance BP is of one of the world’s largest energy companies‚ providing its customers with fuel for transportation
Premium Knowledge management Future Organizational structure
Frankenstein’s Transformation Over the course of Frankenstein‚ Viktor Frankenstein undergoes a drastic emotional transformation as a result of his experiments which resulted in the creation of his Creature. Frankenstein’s trips to Montavert‚ and his descriptions of the scene on his solitary excursions‚ show a clear sense of an emotional ’before and after.’ In his visits to Montavert before the birth of his Creature‚ Frankenstein saw a sublime and beautiful scene. However‚ his accounts are drastically
Premium Romanticism Emotion Mary Shelley
Title of book: Sustaining the Transformation Author: General C.C. Krulak 1. This book is about: This book puts emphasis on the transformation of an American citizen into a marine and the importance of sustaining the change. It is imperative because our war fighting ability depends on a lasting transformation not only during the time of the individual’s Marine Corps career but also when he or she returns to their civilian communities. 2. General plot/story line: The Marine Corps does
Premium Management Leadership Sociology
of the 20th century welcomed a more lenient wardrobe for women as America entered the industrial revolution. The silhouette morphed into the mono-bosom with pigeon front dresses. The legendary Paul Poiret introduced the most notable design of the decade: the hobble skirt. Exceptionally wide at the hips and narrow at the ankles‚ the hobble skirt made walking nearly impossible‚ but fashionable women embraced it nonetheless. Poiret also supported the movement to liberate ladies from the corset; his
Premium World War II Roaring Twenties Haute couture
Despite the fact that the number of employees at Accenture doubled from 2001 to 2008 accenture managed to reduce its spending per employee by 60% and reduce its overall IT expenses as a percentage of revenue by 58%. This was no miracle but a result of strategic business plan that was aligned with the IT. Some of the major problems that the parent firm Anderson faced was of interconnectivity among systems‚ remote access and its offices around the world implemented their own individual accounting and
Premium
Often in novels and stories‚ the main character undergoes a transformation from childhood to adulthood or from youth to maturity. This large change usually occurs through multiple steps in the stories; however‚ there is almost always a single pivotal moment that can be argued as the true moment of the character’s transformation. In The Alchemist by Paulo Coelho‚ the main character‚ Santiago‚ recognizes his purpose in the world. The moment that this happens is when he decides to abandon his current
Premium Meaning of life Debut albums 2007 albums
Energy Transformation Conduction‚ convection‚ and radiation are things that exist in our life. Thanks to that stuff we have things like electricity and things to cook too. I am going to explain things about energy transformation. Heat transfer is the way heat moves through matter to change the temperature of other objects. There are three types of heat transfers‚ Conduction‚ Convection‚ and Radiation. The first kind of heat transfer‚ conduction‚ is heat transferring through direct contact of materials
Premium Heat Energy Temperature