changeDried upNo change 4.sucrose solutionNo changeNo changeDried upNo change 5.starch solutionNo changeDark blue-blackDried upNo change 6.distilled waterNo change (Clear blue)No change (Clear yellow)Dried upNo change Investigation 1A: Testing for Macromolecules Discussion: By observing the table above‚ we could see that Benedict’s test was for reducing sugars‚ iodine test was for the presence of starch‚ filter paper was for the presence of fatty acids‚ and the Biuret test was for amino groups present
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Identification of A Mixed Culture Unknown An experiment such as this one serves the purpose of allowing us‚ the students‚ to apply what we already know about any organism and any laboratory procedure to the difficult task at hand. It is possible to identify a mixed culture by running familiar experiments on the unknown bacteria and taking information already known about specific bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the
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Materials • Samples of different types of milk • Benedict’s Reagent • Biuret Reagent • Sudan III • Water bath • Pipettes/syringes • Test tubes • Microscopic slides and cover slips • Microscope Method (testing for reducing sugars) 1. Add 3cm³ of whole milk‚ by using a pipette or syringe to the test tube. 2. Add 5cm³ of Benedict’s reagent and place it in the boiling water bath for 8 minutes. Do the same for semi-skimmed milk and skimmed milk. 3. Once all 3 of the test tubes are
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Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
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PURPOSEThe purpose of the lab is to learn how to determine the presence of glucose‚ starch‚ lipid‚ and protein in various samples through standard tests. MODIFICATION1. Protein solution‚ vegetable oil‚ glucose solution‚ sucrose solution‚ starch solution and distilled water were added separately into six marked test tubes. The volume added was about one finger thick in depth of a test tube. 2. 5 drops of Iodine solution was added to each test tube. 3. The sample’s reaction with iodine was recorded
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Macromolecules of Life Exercise 1: Testing for Proteins In this experiment Biuret Color is added to four substances‚ egg white‚ pepsin‚ sugar‚ and distilled water to test the detection of protein in each substance. Substance Tested Predicted Results Biuret Color & Number of drops added 1. Egg White Change color 5 drops –turned a yellow/light brown 2. Pepsin Change color 5 drops- turned purple 3. Sugar No Change 5 drops-no change in color 4. Distilled Water No Change 5 drops-no
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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TESTING FOR MACROMOLECULES Describe the positive test for starch. Explain how you know. The positive test for starch is adding iodine solution. In the experiment‚ the starch solution had the most dramatic reaction with the iodine solution. It turned to an opaque bluish-purple solution while other samples all turned to a colour related to orange and yellow other than the vegetable oil. But the vegetable oil was more translucent than completely opaque. Describe the positive test for
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Microbiology-2460 Lab-003 March 31‚2008 Lab Report-Escherichia coli Abstract The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. To obtain the identification of this unknown bacterium‚ several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms. Introduction In a lab today‚ I am to identify an unknown bacterium that is
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Lab Report for Experiment #7 Student’s Name __________Lisa __________ Date of Experiment _08/03/2013__________ Date Report Submitted ______08/05/2013___________ Title: Identification of Metallic Ions Purpose: To perform and observe the flame tests of some alkali and alkaline earth metal ions. Understanding Light Introduction: Electrons from an atom can absorb energy and become excited to move from the stable “ground state” energy level to a higher energy level. Imagine
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