Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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________________________________________ Absorption Alcohol is absorbed from all parts of the gastrointestinal tract largely by simple diffusion into the blood. However the small intestine is by far the most efficient region of the gastrointestinal tract for alcohol absorption because of its very large surface area. In a fasting individual‚ it is generally agreed that 10% to 20% of a dose of alcohol is absorbed from the stomach (the volume of alcohol affects the absorption) and 75% to 80% is absorbed
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chemistry‚ an alcohol is an organic compound in which the hydroxyl functional group (-OH) is bound to a carbon atom. In particular‚ this carbon center should be saturated‚ having single bonds to three other atoms.[1] An important class of alcohols are the simple acyclic alcohols‚ the general formula for which is CnH2n+1OH. Of those‚ ethanol (C2H5OH) is the type of alcohol found in alcoholic beverages‚ and in common speech the word alcohol refers specifically to ethanol. Other alcohols are usually
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ALCOHOLS‚ ETHERS‚ AND PHENOLS Juris Marie G. Garcia Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Date Performed: February 27‚ 2015 Date Submitted: March 06‚ 2015 Answers to Questions: 1.) Balanced equations: A. Lucas Test Test Sample Equation Ethyl Alcohol C2H5OH + HCl + ZnCl2 C2H5Cl + H2O Isopropyl Alcohol (CH3) 2CHOH + HCl (CH3) 2CHCl + ZnC2 + H2O Tert-butyl Alcohol (CH3) 3COH + HCl + ZnCl2 (CH3) 3CCl + ZnCl2 +H2O Benzyl Alcohol C6H5CH2OH + HCl + ZnCl2
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Imran Hyder 4th Period English An Unknown Girl An Unknown Girl is written by the poet Moniza Alvi. Moniza Alvi is a renowned Pakistani-British writer. Her mother is British and her father is from Pakistan. She was born in Pakistan and later moved to the United Kingdom. This could reflect why An Unknown Girl is about the struggle Alvi or The Unknown Girl has trying to relocate her cultural identity and cling onto it in a nation where the culture is slowly drifting away and being replaced by western
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Kevin Warwick’s “Into the Unknown‚” he argues that instead of measuring intelligence in a single dimensional value‚ difference aspects like culture and values should be taken into considerations throughout the process. In “The Social Imagination‚” James Flynn expresses a similar viewpoint on the study of intelligence‚ claiming that psychologists tend to overlook and underestimate the social contexts that could possibly alter the results of
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How much alcohol do teens use? Alcohol is the most frequently used drug by teenagers in the United States. About half of junior high and senior high school students drink alcohol on a monthly basis‚ and 14% of teens have been intoxicated at least once in the past year. Nearly 8% of teens who drink say they drink at least five or more alcoholic drinks in a row (binge drink). What are the dangerous effects of alcohol use in teens? Just a few of the many dangerous effects of alcohol use in teens
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is green‚ when an alcohol is oxidized by the reagent. A change in color of the reagent from orange to green represents a positive test. Primary alcohols are oxidized by the reagent to carboxylic aicds; secondary alcohols are oxidized to ketones. Tertiary alcohols are not oxidized at all by the reagent. Hence‚ this reaction can be used to distinguish tertiary alcohols form primary and secondary alcohols. Procedures: 1. Place ethanol‚ isopropyl alcohol and tert-butyl alcohol in three different
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Alcohol Facts and statistics ( www.niaaa.nih.gov-2012 ) Global Burden of alcohol abuse‚ In 2012‚ 3.3 million deaths‚ or 5.9 percent of all global deaths (7.6 percent for men and 4 percent for women)‚ were attributable to alcohol consumption.. Alcohol contributes to over 200 diseases and injury-related health conditions‚ most notably alcohol dependence‚ liver cirrhosis‚ cancers‚ and injuries. In 2012‚ alcohol accounted for 5.1 percent of disability adjusted life years (DALYs) worldwide. Globally‚
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