The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
Premium Laboratory glassware Laboratory equipment Chemistry
University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
Premium Protein Chromatography Analytical chemistry
Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
Premium Agar plate Growth medium Bacteria
Abstract: This report presents the reason why the ocean does not freeze in the winter. Since the ocean consists of salt water‚ an experiment was done to test the effect of freezing on salt water compared to pure water. The experiment was conducted three times in order to obtain accurate results and eliminate errors. In addition to the salt and pure water experiment‚ a variation of this experiment was created to test other options. In the variation experiment the effect of freezing on sugar water
Premium Water
the percent yield by dividing the expected yield‚ the amount of product that should be produced based on your stoichiometric calculations‚ by the actual yield‚ the amount of product that is experimentally obtained from a chemical reaction. In this lab‚ I have determined the reaction for mixing two reactants together; I measured out 0.005 moles of each reactant‚ lead (II) nitrate and potassium chromate. I dissolved‚ mixed‚ and made them react to make products; I compared the mass of the two reactants
Premium Stoichiometry Chemical reaction
Flame Test Lab Morgan Chemistry CP 5/30/14 Abstract- In the flame test lab‚ the flame test was performed to excite the electrons in the samples and observe the color of the flame. The flame emits a color because each element has an exactly defined emission spectrum‚ which one can use to identify them. For example‚ NaCl was highlighter yellow‚ Sr(NO3)2 was sun orange‚ CuCl2 was turquoise‚ LiCl was neon red‚ KCl was solar flare yellow‚ and BaCl2 was Voldemort green. Introduction- In Bohr’s
Premium Chemistry Potassium Gas
Lab report for Experiment #2: Extraction Your Name: Name of TA: Lab Partner’s Name: Lab Section: Title: Experiment #2: Extraction Purpose: What is the purpose of this lab? In your OWN words! Observations: Weighed out 3.2568 grams of chemical mixture that was yellow in color. Dissolved dry chemicals in 38 ml CH2Cl2 with gentle heating. Poured the yellow solution into sep funnel. Added 10 ml CH2Cl2 to flask to rinse‚ poured solution into sep funnel. Added 15 ml 3 M
Premium PH Solubility Chemistry
Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential
Premium Gram staining Staining Microbiology
everyday lives. Many artists today even try to mimic artists in ancient Greece‚ but often fail to do so. One of the most important aspects to come out of Greek art is the architecture‚ most notable the three architectural orders of ancient Greece‚ Doric‚ Ionic and Corinthian. Each components of this order has a system of inter dependable parts whose proportions are based upon mathematical ratios. The basic components of all three orders are the column and entablature. Although most people do not know this
Premium Doric order Ionic order Corinthian order
Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a & b) are
Free Electromagnetic radiation Light Spectroscopy