Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology Dissecting set
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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Blue on Agar Plates at Different Temperatures Abstract: Diffusion is the movement of particles from an area of high concentration to an area of low concentration wherein substances are independent‚ rapid and in random motion which frequently collide with each other in the sides of the container. This study aimed to assess the influence of temperature on the diffusion rate of methylene blue on agar plates. To determine the relationship between the two variables‚ five set-ups of agar plates were prepared
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Problem What effect does the different concentration of hydrochloric acid have to the rate of diffusion of agar that is dissolved with sodium carbonate and phenolphthalein? Introduction Diffusion is a process where the molecules intermingle as a result of their kinetic energy of random motion and is the net movement of like molecules down their concentration gradient‚ which is the energy inherent in their constant molecular motion makes them collide and careen outward from the region of higher
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MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed‚ there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is
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having a universal method of identifying bacteria allows for all scientists from any part of the world to identify the same species in an identical manner allowing for a precise of classification. Bacteria are distributed throughout the world in almost every conceivable habit. Bacteria are unicellular microorganisms‚ with variable shapes and nutritional needs. They lack a distinct nucleus and occur singly or in chains or clusters and form distinct colonies. Bacteria are classified on the basis of
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Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated‚ why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time‚ propose is to isolate the unknown bacteria. Therefore‚ the first time to streak on the plate‚ there are million of bacteria on the loop. For that reason‚ we need to sterilize
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Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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bacterium to be identified as a result of this study. This study allows the student to exercise their ability to use and understand sterile techniques‚ transmission‚ and prevention of microorganisms. Materials provided by the instructor‚ including an agar plate and cotton swab were used to collect
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BACTERIA Period: 4 Characteristics: 3 major shapes Cocci Basilli Spirilla 3 major components Mesosomes flagella Plasmids Growing Up: Bacteria can obtain energy through phototrophs(sunlight)‚ lithotrophs(inorganic compounds)‚ and organotrophs(organic compounds) Marriage/Reproduction Binary Fission: The process by which all bacteria reproduce. It results in the separation of a single cell into two. Transformation: genetic alteration
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