CHAPTER 1 INTRODUCTION One of the most common infections that we can easily get was skin infections caused by bacteria‚ specifically Staphylococcus aureus and Staphylococcus epidermidis. These two Staphylococcus species can found worldwide‚ they are a small component of soil microbial flora. We have different treatments for skin infections‚ but in those treatments only few people can afford it. Because most of them are expensive for ordinary people who are usually
Premium Infection Bacteria Staphylococcus
the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies
Premium Bacteria Microbiology Escherichia coli
Optimal Growth Conditions and Generation Time for E. coli Introduction The goals of these exercises were to determine the optimal growing condition and the generation time of E. coli. Materials and Methods Please refer to the lab manual for a complete list of materials. To determine the optimal growth condition for E. coli‚ we had to test the environmental factors that could affect the growth. We tested the relative growth of E. coli in the presence and in the absence of oxygen. The results
Free Bacterial growth Temperature Celsius
QUEENSLAND ACADEMIES FOR SCIENCE‚ MATHEMATICS AND TECHNOLOGY International Baccalaureate Diploma Program Extended Essay in Biology Is Tea-tree Oil effective in controlling and minimising the growth of the Escherichia coli Bacteria? A study of the effectiveness of a Natural Antiseptic Candidate Name: Lois Adrienne Dianne Soriano Dulay Candidate Session Number: 003072-032 Word Count: 3997 Supervisor: Ms Kate Barry Date of Submission: 25th July 2011 Acknowledgments Firstly I would
Premium Escherichia coli Bacteria Tea tree oil
on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials
Premium Staphylococcus Bacteria Staphylococcus aureus
Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
Premium DNA Gene Molecular biology
Methicillin-Resistant Staphylococcus aureus (MRSA) Nasal Colonization and Infection Isolates in a Veterans Affairs Hospital Staphylococcus aureus (S. aureus) belongs to the genus Staphylococcus. It is Gram-positive‚ cocci shaped anaerobic bacteria that forms clusters and produces catalase‚ which catalyzes hydrogen peroxide to water and oxygen. It contains a cell wall structure (including peptidoglycan and teichoic acid) and a low G + C content of DNA between 30-40 mol %. (Bauman‚ R.‚ & Masuoka‚ E. (2015). Microbiology:
Premium Bacteria Antibiotic resistance Immune system
Carlie Haeffner Qualitative Analysis March 20‚ 2015 The purpose of this lab is to identify unknown ions in a solution by using a type of chemical reaction called precipitate reactions. The key to finding which ions are present in a solution is to form a precipitate which makes the ions “come out” in a reaction (McNeil‚ 2013-2014). Water is used in these experiments to act as a solvent. Since water is a polar molecule‚ the slightly positive and negative charges will sometimes pull apart molecules
Premium Chemistry Water Experiment
Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent
Premium Bacteria Gram staining Staining
Double unknown identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive
Premium Bacteria Microbiology Gram staining