MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed‚ there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is
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1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a sample of different
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Staph infections are caused by a staphylococcus bacterium‚ Staphylococcus aureus. S. aureus is a purple‚ cocci shaped cell with its morphological arrangements in grape-like clusters‚ it is determined by a bacteriological stain‚ known as the Gram stain. This determines it to be a gram positive bacterium. When grown on bacteriological media‚ S. aureus appears as a large white to golden colony. It is not very fastidious but it grows well‚ either aerobically or under anaerobic conditions and produces
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Escherichia coli is a rod-shaped Gram-negative bacteria from the Enterobacteriaceae family‚ which owns the usual flora and can be be as pathogenic Human IN DUE can be cause diarrhea. The rational NOT USE ON antibiotics Infectious diseases can be causing the occurrence of resistance so that the necessary alternative to using Active Ingredients antimicrobial From medicinal plants. Turmeric (Curcuma domestica Val.) It is a prayer One Crop Growing in Indonesia That can be used as a medicinal plant. BECAUSE
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microorganisms. -Gram stain assists in identifying the type of microbes‚ whether they are gram positive or negative‚ which in turn tells us what kind of cell wall the microbes have. -Endospore stain tells whether microorganisms make endospores. -Acid-fast stain is helpful in recognizing the existence of mycolic acid in cell walls. -Capsule stain reveals the existence of capsules. Methods: Simple Stain For this experiment‚ I used E. coli to make a heat fix smear. I placed a
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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My journey to the unknown started about three weeks before the end of my microbiology class at Brunswick Community College. This journey started out with a media plate cultured with an unknown species of bacteria. I was then instructed by my professor to use all of my previous knowledge to identify the unknown bacterial pathogen. This lab led me to master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with
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IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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the need to identify an unknown microbe may arise. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. We embarked on the project with the knowledge that we would have to identify a Gram(+) as well as a Gram(-) from the species that were given and the
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