Material Appendix B – Identifying Bacteria Bacteria identification is accomplished in a number of ways. Two common tools microbiologists use to identify unknown bacteria include dichotomous key and biochemical tests. The dichotomous key is useful when a microbiologist only needs to know which group an unknown microbe belongs to on a general level. When a microbiologist needs to identify a specific bacterium‚ biochemical tests are used. PART ONE: GENERAL BACTERIA IDENTIFICATION Review the
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Gram Positive Unknown: “Enterococcus faecalis” Family: enterococcaceae Genus: enterococcus Species: faecalis Gram + Oxygen class: facultative anaerobe Temperature class: mesophile – they can grow in the range of 10 °C - 45 °C pH class: can grow at a pH range of (4.6 – 9.9) with the optimum at 7.5 Enterococci can survive very harsh environments including extreme alkaline pH 9.6 and salt concentrations (basic). Environment: They can
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known as E. coli and 0157:H7; E. coli found in the human intestinal tract and is found in multiple forms. E. coli is rod shaped and the size of two microns to one micron. Widely known for the deadly stain found in feces and meat. The pathogen is still a major instrumental issue in human health and is easily infectious with contaminated sustenance. E. coli is gram-negative bacilli tested in clinical settings from stool specimens. The tests detect the presence of disease causing pathogens of E. coli
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Objective: To successfully separate and identify all three unknown cations‚ found in a mixture and belonging to one of two specific groups (2 from one and 1 from the other). In order to do this we will be using various tests and reactions and observing the effect they had on our solution. Materials: Pasteur pipets Bunsen Burners Stirring rods Excess reagents Hot plates Sample QA unknown 101-5.7 Table of steps and observations: Step/Test |Observation |Inference | |2-1
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Streptococcus faecalis Streptococcus faecalis also known as strep D‚ is now known as Enterococcus faecalis. Enterococcus faecalis is part of the Enterococcaceae family. This organism is a gram positive and usually occurs in pairs called diplococci. It is facultative anaerobic‚ and is nonmotile. This organism is a lactose fermenter and can be grown in 6.5% NaCL. Enterococcus faecalis is considered non-hemolytic‚ meaning it does not break down blood cells. Enterococcus faecalis is part of normal
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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Microbiology UNKNOWN LAB REPORT Kateryna Petrakova INTRODUCTION Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and‚ therefore‚ to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and
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_____________________________________________ Project 3: Identifying the Unknown White Compound by Testing Physical and Chemical Properties _____________________________________________ Michigan State University Chemistry Lab 161 Section 27 19 October 2017 Friday 11:00-1:50 Introduction For this experiment‚ there was an unknown white compound that had to be identified. By testing this compound for different properties it made making an educated guess easier to determine
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Carlie Haeffner Qualitative Analysis March 20‚ 2015 The purpose of this lab is to identify unknown ions in a solution by using a type of chemical reaction called precipitate reactions. The key to finding which ions are present in a solution is to form a precipitate which makes the ions “come out” in a reaction (McNeil‚ 2013-2014). Water is used in these experiments to act as a solvent. Since water is a polar molecule‚ the slightly positive and negative charges will sometimes pull apart molecules
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Enterococcus faecalis is a gram positive‚ commensal bacterium inhabiting the gastrointestinal tracts of humans and other mammals [1].E. faecalis can cause life-threatening infections in humans. Moreover‚ the antibiotic resistance exhibited at high levels by E. faecalis contributes to its pathogenicity [2]. Several reports state that enterococcal species possess the property of causing a variety of diseases in humans [3‚ 4]. It is known from previous studies that Enterococcus faecalis can cause endocarditis
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