Effect of Osmotic Stress and Temperature on Microbial Growth BIO 3400-002L – Microbiology Lab 1 Effect of Osmotic Stress and Temperature on Microbial Growth Luiz Felipe Isidoro ABSTRACT Evolution allowed primitive forms of life to develop proteins and enzymes that made it possible for life to evolve under environments with hostile conditions‚ such as high salt and heat. More specifically‚ some bacteria selected genes that code for peptides with stronger intermolecular forces‚ coping
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hypothesis that: Osmosis is dependent on the concentrations of the substances involved. Diffusion is the passage of solute molecules from an area of high concentration to an area of low concentration (Campbell & Reece‚ 2005). An example is ammonia diffusing throughout a room. A solute is one of two components in a chemical solution. The solute is the substance dissolved in the solution. The solvent‚ the other component‚ is any liquid in which the solute can be dissolved (Anderson‚ 2002). Diffusion
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2. Draw and completely label a cell membrane. [pic] 4. List and briefly discuss the 5 components of cellular membranes. 1. Phospholipids layer- 2 layers ‚ polar and non polar ‚ polar facing the extracellular fluid and cytoplasm 2. Transmembrane proteins-integral membrane proteins are proteins that expand the membrane 3. Interior protein network-peripheral proteins are in the interior side of the membrane 4. Cell surface markers are glycolipids‚ carbohydrates/lipid
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Name: ____Justine Basilone___ Osmotic Regulation Virtual Laboratory Biology Go to the following virtual laboratory: http://www.glencoe.com/sites/common_assets/science/virtual_labs/LS03/LS03.html Answer your questions in this word document‚ save the file and submit through Edmodo. Your answers should be at least 2 complete sentences if not more! Part I: Observations & Data: Read the Introductory information and the Procedure located in the window to the left of the screen and
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PLASMA MEMBRANE SELECTIVE PERMEABILITY= allowing some substance to cross it more easily than others composed of: Phospholipids Proteins Carbohydrates Cholesterol FLUID MOSAIC MODEL Phospholipids are primary lipids(constantly moving‚ fluidly) AMPHIPATHIC-containing hydrophobic and hydrophilic regions Mosaic part=not made of one thing Freeze-fracture studies:way to view what’s inside FLUIDITY OF MEMBRANE Move within bilayer Most of lipids and some proteins‚ drift laterally
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Cell Membranes and Transport Hands-On Labs‚ Inc. Version 42-0033-00-01 Exercise 1: Diffusion Observations Data Table 1: Rate of diffusion in different temperatures | | ºC | Minutes | Temperature | InitialTemp. | InitialColor | 5 | 10 | 15 | 20 | 25 | 30 | 60 | Cold | 10°celsius | clear | clear | clear | clear | clear | clear | light purple | light purple | Ambient | 25°celsius
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Topic 2 – Cell membranes Reading Chapter 5 Objectives Functions of cell membranes The fluid mosaic model The lipid bilayer – phospholipids‚ glycolipids and sterols Properties of the lipid bilayer Membrane proteins – the ‘mosaic’ in the model Solute movements across membranes BIO 1140 – SLIDE 1 http://www.nikonsmallworld.com/gallery/year/2006/id/751 Functions of cell membranes – an overview Fluid mosaic model Singer & Nicolson 1972 Fluid lipid molecules in which
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Lab activity 1: Effect of temperature‚ concentration‚ and pressure on equilibrium Introduction Our ongoing discussion has been on systems at dynamic equilibrium: for a reversible reaction‚ the rate of the forward reaction is equal to the rate of the reverse reaction. What happens if equilibrium is disturbed? In this lab activity‚ we are going to examine the effect of changing reaction conditions on the position of equilibrium. Part I: Effect of temperature We will consider the equilibrium
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EFFECT OF CHANGES IN TEMPERATURE AND SDS CONCENTRATION ON THE STRUCTURE OF BEETROOT CELL MEMBRANES. Introduction Cellular Membranes or cell membranes are biological membranes that separate the interior of a cell from the outside environment. Cell membranes are selectively permeable to ions and some molecules and control the movement of substances in and out of the cell. (Danyk‚ 2013/14) Cell membranes separate and organize chemicals and reactions within cells by allowing selective passage
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Biology Experiment 2 : Transport Across Membrane Name : Umi Adiba Anis Binti Che Hamid Matrix Number : CPM0147_2013B Batch : Pre-Med May Intake 2013 Group : E Date : 19.06.2013 Lecturer’s Name : Miss Nurul Izziani Objectives : I. To study the effects of hypotonic‚ hypertonic‚ and isotonic solutions on plant and animal cells. Experiment 1 : Introduction : The purpose of doing this experiment is to investigate the effects of hypotonic‚ hypertonic‚ and isotonic solutions on plant
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