Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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BRADFORD ASSAY Calculation Formulas: Absorbance of BSA = A595 nm Raw Data = Average of A595 nm of three wells ÷ 3 Example: A1+A2+A3 ÷ 3 In this case‚ 0.365+0.374+0.453 ÷ 3 = .397 Corrected Data =( (A595 nm well) – BackGround) or (Raw Data – Background) Background = negative control = Bradford Reagent + No Protein Background of this standard curve = A595 nm of well ‘A’ = .397 We used well ‘A’ as our negative control. DATA RAW DATA
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Introduction to IMMUNOLOGY COURSE • Subject Agenda* Theoretical part (Lecture): 14.01----18.03----22.04.2013 Practical part (Labwork) • Study Materials: Textbook (David Male and Ivan Roitt-2006-DIR; Abul Abbas-2007AA)‚ Clips and Internet search • Classconversation@gmail.com. Pass: btiu12345 • Evaluation - Midterm Exam‚ Final Exam‚ Labwork - Assignment (Home-work‚ Topic-oriented-In-class discussion‚ Readand-Present Practice) Contact me at: R501‚ IU Building; or via email: ntthoai@hcmiu.edu
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Title: Immunology Aim: To visit a microbiology department at the university hospital and acquire detailed information of various antigen-antibody tests eg. ASO‚ haemotoglutination‚ ELISA etc. Method: To listen to presentation made and thus formulate a concise report on that which was discussed and demonstrated. (Please see BC34D Laboratory Manual for more information) Concise Report/Summery: The term serology refers to both the study of general antigen-antibody reactions in a laboratory
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85326 Lecture 5 Role of transmembrane signaling in immunorecognition (signal transduction) G Ligand (primary 0 messenger) 0.0 shapeType75fBehindDocument1pWrapPolygonVertices8;18;(8674‚879);(15125‚879);(15125‚145);(21500‚145);(21500‚1540);(14509‚1540);(14509‚21497);(3531‚21497);(3531‚19296);(3607‚19296);(3607‚15187);(2071‚15187);(2071‚2713);(0‚2713);(0‚0);(3223‚0);(3223‚806);(8674‚806)posrelh0posrelv0pib Receptor-ligand binding CYTOSOL Cellular responses Signal
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Unit 10: Blood/Immunology Case Study Lab What were your three diagnoses? Case 1: Bacterial or viral infection Case 2: Normal blood smear Case 3: Normal blood smear Journal Questions for lab In what ways do normal red and white blood cells differ? White blood cells have a nucleus‚ red blood cells do not‚ white blood cells protect while red blood cells transport‚ red blood cells have a longer life span than white blood cells. Which type of white blood cell would you
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Innate Targeting of Pathogens Pathogen Examples Facts Pattern recognition alerted by Viruses flu‚ small pox‚ HIV‚ polio‚ Ebola‚ rhinovirus‚ hepatitis‚ measles Can only reproduce inside cells Foreign nucleic acids (double stranded RNA‚ single stranded DNA‚ foreign methylation patterns)‚ reduced antigen presentation by infected cells So viruses are usually recognized by something unusual in their nucleic acids.(Double RNA‚ normally‚ RNA is a single strain) Bacteria
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Papa Kwame Pireku Problem set 1 2.2) Antimicrobial peptides are usually produced as inactive propeptides that require cleavage by a protease to complete their activation‚ often by generating a cationic peptide that takes on an amphipathic structure capable of disrupting the cell membrane of a microbe. Also in neutrophils the antimicrobial agents are activated by proteolytic cleavage when these granules fuse with phagosome and encounter neutrophil elastase that has been released from primary granules
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NEUTROPHILS & MACROPHAGES: Neutrophils & Macrophages play the main role in (SEEK AND DESTROY) the invading agents (microbes‚viruses and other injurious agents) NEUTROPHILS are mature cells that can SEEK AND DESTROY agents even in BLOOD ‚ MACROPHAGES begin their life as immature cells in blood called monocytes ‚ when they enter the tissues they swell and become mature and named MACROPHAGES which are extremely capable of SEEK AND DESTROY intratissue injurious agents. White Blood Cells Enter the
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Unit 10: Blood/Immunology Case Study Lab What were your three diagnoses? 1. Case 1: Pernicious Anemia 2. Case 2: Normal Blood Smear 3. Case 3: Malaria Journal Questions for lab 1. In what ways do normal red and white blood cells differ? Many red blood cells circulate in the bloodstream‚ while the white blood cells leave the blood. 2. Which type of white blood cell would you expect to be most common in a normal blood smear? Neutrophils 3. A differential
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