With my specific water sample‚ I then transferred 10 ml of the sample water to the five double strength broths‚ 1.0 ml to five of the single strength‚ and 0.1 ml of water to the other five. After incubating them for 24 hours‚ I observed each tube to see if they obtained a bubble and changed from yellow to red. If the tube turned red it showed it was positive for gas production‚ and if there was a color change then it indicated the production of acid
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tip for each transfer of DNA and enzyme (refer to figure 6.1). The solutions were covered and mixed gently by tapping the reaction tubes on the table which then allowed for the contents to assemble at the bottom of the reaction tubes‚ followed by incubating the reaction tubes in a 37°C waterbath for 45 minutes. Finally‚ after the incubation was complete‚ 5 µl of 10x gel loading solution was added to each reaction tube to stop the reaction. Once again‚ the reaction tubes were covered and tapped gently
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3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
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DEVELOPMENT OF SORBITAN MONOSTEARATE ORGANOGELS FOR CONTROLLED DELIVERY SYSTEMS A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF Bachelor of Technology (Biomedical Engineering) Submitted By MEENAKSHI SINGH Roll No. 107BM008 Under the Guidance of Dr. Kunal Pal Department of Biotechnology & Medical Engineering National Institute of Technology Rourkela 769008 DEPARTMENT OF BIOTECHNOLOGY & MEDICAL ENGINEERING‚ NATIONAL INSTITUTE OF TECHNOLOGY-ROURKELA
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citrate buffer‚ pH 5.0 and was incubated at 37° Celsius for two hours to allow for the enzyme to digest the glycogen. Sample B was the control‚ where only .2M citrate buffer‚ pH 5.0 was added which was used to measure the amount of free glucose. While incubating‚ glycerol was determined by using 3 ml triglyceride reagent incubated at 37°celsius for 5 minutes. After warmed‚ 30 microliters of homogenate was added to
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------------------------------------------------- Audio-Visual Presentation Unified Document Group 4: “Lugue Kayo!” Topic: “Using antibody-antigen interaction for diagnostics and other purposes” Members and Fields of Study: Mark Louie Lugue – Scientific Interaction of Antigens and Antibodies Interaction Used in Diagnostics James Pe Lim – Pros and Cons of this Biotechnology Ria Iestin Corral – Antibody and Antigen Descriptions Hessen Demil Jao – Interaction
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Respiration of Sugars by Yeast 1. PROBLEM STATEMENT: What is the effect of the type of sugar on the amount of carbon dioxide released by Yeast during aerobic respiration? 2. HYPOTHESIS: If the type of sugar is changed‚ the amount of carbon dioxide created will then increase because sugar is needed to for respiration so occur. If Fructose is added to the Yeast it will then respire the most Co2 because fructose is the largest sugar‚ its like using a hundred dollar bill instead of single dollars
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2.2 Immunoprecipitation Different protocols were compared so as to choose the one which is compatible with the characteristics of the particular protein of interest [29]. A suitable amount of nuclear extracts was diluted with 100 microliters of chilled PBS‚ 0.1% Triton 100 (to this mixture‚ the following was added to make a final concentration of 1:1000: 0.2 mM PMSF‚ 0.5 mM DTT and 15 (microgram/ microliter). It was made sure that the final concentration of nuclear extract was 0.5-1.0(microgram/
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Do you know of a qualified avian or other vet close to you for help when you need it? Your closest avian vet should be one of your birds best friends. They are the ones you will need to go to when you have a problem and often times it will be a life threatening one when you are faced with rushing them to one. If you do not have an avian vet in your area‚ please find the closest other vet that at least has some idea of how to care for and treat birds. Any vet is better than none at all! Here
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1.1 and 1.2 Bacteria - are micro-organisms that consist of only one cell. Bacteria multiply by splitting themselves in two‚ which is called a binary fission. Because of this they can increase in number rapidly. The majority are harmless‚ but some can be pathogenic which results in bacterial infection occuring. Bacterial infections can be treated by using antibiotics. Bacteria can evolve a resistance to antibiotic e.g. MRSA. Some diseases caused by bacteria include tuberculosis‚ pneumonia‚ salmonella
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