Abstract Three tests were performed to determine the identity of unknown number 34‚ a gram stain‚ citrate utilization test‚ and a urease detection test. These tests are explained in detail‚ and the method of performing the tests is described. The tests showed that the bacterium was gram-negative bacilli that did not utilize citrate‚ and the ability to hydrolyze urea using urease. These results indicate that the identity of unknown number 34 is Proteus mirabilis. Introduction Proteus mirabilis is
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Introduction: Homeostasis is the tendency of the body to seek and maintain equilibrium‚ a state of balance‚ within its internal environment. Endotherms are “warm blooded” animals that produce and maintain their own internal body heat. Most mammals and a few birds‚ are endotherms; this is beneficial because the enzymes in the body that carry out metabolic processes have an optimum temperature at which they function‚ having the body constantly be‚ or be close to‚ this optimum internal temperature
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Introduction Transformation is a genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surrounding through the cell membrane. The arabinose operon changes AraC from a repressor to an activator; in this experiment the pGLO plasmid has been designed with a modified operon so that in the presence of the arabinose the bacterial cells which have been transformed by the pGLO plasmid will fluoresce due to the production of GFP. SDS-PAGE
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Effect of Varying Temperatures: The enzyme catalyzed reaction rate during varying incubation temperatures are plotted on Figure. 6. As the temperature increases the rate increases‚ but as the temperature reaches 49oC it begins to drop. When the plot of the logarithm of the rate is used against the inverse of the temperature kelvin’s the Arrhenius equation is used to calculate the activation energy. The range in orange is between 16.5 - 37oC and the activation energy is calculated to be 9332kcal/mol
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Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
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Kate Chopin’s “The Awakening” examines the implications placed on women for self expression during the 1800’s. Banned for several years by critics after its initial publication in 1899 because of its unsettling content‚ “The Awakening” later became a most cherished account of a woman’s journey towards self-discovery and abandonment of her conventional society. (Kester-Shelton) Within that story is where we meet Robert LeBrun‚ A young‚ flirtatious and confident womanizer with a reputation to match
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Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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The samples were boiled for 3 minutes and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed
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BIO 5 Lab Report: Lactase Enzymes Enzymes are biological catalysts or assistants. Enzymes consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. Enzymes can either launch a reaction or speed it up. The chemicals that are transformed with the help of enzymes are called substrates. In the absence of enzymes‚ these chemicals are called reactants. Enzymes are thought to have an area with a very particular shape. When a molecule of
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What Is Soil Pollution? [pic] What Is Soil Pollution? Soil pollution results from the build up of contaminants‚ toxic compounds‚ radioactive materials‚ salts‚ chemicals and cancer-causing agents. The most common soil pollutants are hydrocarbons‚ heavy metals (cadmium‚ lead‚ chromium‚ copper‚ zinc‚ mercury and arsenic)‚ herbicides‚ pesticides‚ oils‚ tars‚ PCBs and dioxins. Until the 1970s‚ there was little talk of soil pollution and its devastating effects. In the 1980s‚ the U.S. Superfund was
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