Cloning of plasmid pUC19 in E.coli bacteria Introduction One aspect of the DNA cloning experiments that is carefully considered is the selection of cloning vectors. A variety of vectors have been created‚ each being suitable for a particular use. One common vector used in laboratories is a plasmid called pUC19. It is 2686 base pairs long and possesses an origin of replication which allows the production of over 100 copies in a competent E.coli cell. It possesses a multiple cloning site (MCS)
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1. What are the main benefits of Coffee-Mate and what is limiting its sales? First of all‚ Coffee-Mate´s main benefit is its ability to replace cream or real milk. Furthermore‚ it can be stored for a much longer time than milk or cream making it a good substitute. People who cannot drink coffee without milk don’t need to carry around or look for milk since coffee mate will do the same job. In addition it is made of health promoting ingredients such as dried glucose and vegetable fat. However
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Nicole Hain Bio 110 Sordaria fimicola Lab Report Introduction “Evolution Canyon” consists of two slopes in Israel that are close in proximity‚ but have a huge difference in environment. This makes the slopes a good model system for exploring evolution‚ especially because they’re so close to one another. The South Facing Side (SFS) receives more sunlight than the North Facing Side (NFS). This extreme sun exposure causes the South slope to have drought and arid conditions whereas the North side has
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Arianna Montoya Osmosis and Diffusion Lab Report Background Information: Diffusion involves the movement of solute particles across a selectively permeable membrane from higher to lower concentrations. A selectively permeable membrane allows only some thing to go through. Water and oxygen are examples of molecules that are able to go through the membrane
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Are you using manual hexadimethrine bromide (polybrene) test in blood cross matching? Watch out! Abstract: BACKGROUND: Blood cross matching test mainly includes manual hexadimethrine bromide (polybrene) test (MPT) and the automatic analyzer method (by coombs card) at the Department of blood transfusion. Generally instrumental method is used for large quantities of samples. It can save manpower; MPT is used for emergency and few samples. it can save time. Our department recently encountered a case
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Title: Investigation of Induction Time of lac operon in E. Coli with IPTG‚ and Comparision of the Amount Beta-galactosidase produced with Lactose‚ IPTG and Antibiotic. Abstract: Introduction: An operon is a group of genes that are arranged side by side with a regulatory gene. It also includes a promoter gene‚ operator gene‚ structural genes. Regulatory genes control transcription with positive or negative signal. (Jacob‚ and Monod‚ 1961) A positive signal‚ inducer‚ would stimulate
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Gonorrhea and its Causative Agent; a study of challenges of treating gonorrhea By: Nader Hossaini George Mason University Word count: 2281 Gonorrhea and its Causative Agent Abstract In this study‚ gonorrhea which is a sexually transmitted disease that can be found in every parts of the world including in the United States was investigated; gonorrhea passes from one person to another mainly through unprotected sexual activity. The causative agent for the disease
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Human power-based energy harvesting strategies for mobile electronic devices a. Describe the application (10%) 1.2.3 Wearable or implantable medical devices Implantable or wearable medical devices refer to any device that could help monitor metabolic parameters‚ assist defective physical function or cure diseases. Structural devices such as artificial joints‚ vascular grafts and artificial valves are called passive devices and their working does not need external power. But active
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IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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