1. To determine the wavelength of maximum absorption‚ Amax of bromophenol blue. 2. To construct a standard concentration curve for bromophenol blue. 3. To determine the concentration of the unknown bromophenol blue solutions. 4. To determine the concentration of two different solutes‚ bromophenol blue and methyl orange‚ in a mixture. Material and method: Refer to practical manual page 7 Results: Part 1: Determination
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Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Laboratory Report: Flame Colors of Metals Abstract The experiment about flame color of metals demonstrates how electrons jump into their excitedstate from their ground state as it came in contact with heat. As it return to its lower energy state‚it emits the absorbed energy in the form of light. A platinum or nichrome wire was dipped into aconcentrated HCL and was heated to clean it. The wire was dipped into different salt solutionsand heated in order to determine the metallic ion’s color and wavelength
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REFERENCES 3. DEFINITION 4. PERSONNEL QUALIFICATION 5. GENERAL REQUIREMENTS 6. SAFETY 7. EQUIPMENT 8. APPLICATION FOR PMI 9. PROCEDURE 10. ACCEPTANCE STANDARD 11. REJECTION PROCEDURES 12. REPORT 13. MARKING AND COLOR CODING 14. ATTACHMENT BQO" " Jotqfdujpo" " Joufsobujpobm" " Dp/-"Mue/f 28 Feb.‚ 1 2011 of 16 INSPECTION PROCEDURE POSITIVE MATERIAL IDENTIFICATION PROCEDURE Doc. No. APN – PMI – S – 001
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Introduction In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role‚ if any‚ that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose‚ lactose will separate into galactose and glucose‚ as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions‚ and
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In this lab the densities of unknown irregularly shaped solids and liquids was determined. Accuracy and precision of the results will be estimated‚ and attention will be paid to the correct use of the significant figures. The experiment approach that will be used will tell the mass and volume of the metal and liquid determined by measuring these two quantities with a graduated cylinder and biuret. Procedure: a) The density of metals First I obtained a quantity of unknown metal. Recording
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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